LOCUS       sequence-78430-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN401
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(253..273)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(280..345)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(529..575)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(564..584)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 attcttcctg cgcgtgtacc gcctgcgggc ggtgcgcttg agccgcgtag cctcagccgt
       61 ctcgccgctg tcgaacagga gagcgccaat gagattcttc ttgatggaat gccgatcggt
      121 gttgcccagg accttgaact tctttgaggg caccttgtac tcgtcggtga tcacggccca
      181 gccaacagag ttagtcccaa tatcgaggcc gatcgagtac ttcttgtcag cagctggcac
      241 cccgtggatg ccaaccttcc tcttcttctt cggagccatc ttgtcatcat catccttgta
      301 atcaatgtcg tggtccttgt aatccccgtc gtggtccttg taatccatct agagcggccg
      361 cggccataca agtattgggg atccccctcg agtatcgttc gtaaatggtg aaaattttca
      421 gaaaattgct tttgctttaa aagaaatgat ttaaattgct gcaatagaag tagaatgctt
      481 gattgcttga gattcgtttg ttttgtatat gttgtgttga ggtcgaggtc ctctccaaat
      541 gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat
      601 cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt
      661 cttctttttc cacgatgctc ctc
//
