LOCUS       sequence-78431-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (AtU6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN401
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..41
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact
       61 gaaggcggga aacgacaatc tgatccaagc tcaagctaag ctcacgtgac ggaattaagc
      121 ttcgacttgc cttccgcaca atacatcatt tcttcttagc tttttttctt cttcttcgtt
      181 catacagttt ttttttgttt atcagcttac attttcttga accgtagctt tcgttttctt
      241 ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc caggattaga
      301 atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat tcttaagata
      361 tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg cccatttata
      421 tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca atcttcaaaa
      481 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta
      541 gtgattggga gaccaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
      601 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      661 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
      721 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
      781 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
      841 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
      901 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
      961 agtgatattg atttgctggt tac
//
