LOCUS       sequence-78432-e        2817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human codon optimized Alpha-synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2817)
  TITLE     MJFF plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: human Alpha-synuclein
FEATURES             Location/Qualifiers
     source          1..2817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      392..401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(960..977)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1070..1658)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1150..1169)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1829..2620,2621..2689))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2452..2471
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2690..2794)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag agctcaccgg tgccaccatg gacgtgttca tgaagggcct
      421 gagcaaggcc aaagagggcg tggtggccgc tgccgaaaag accaaacagg gggtggccga
      481 agccgctggc aagacaaaag aaggcgtgct gtacgtgggc tccaaaacaa aagagggggt
      541 ggtgcacggc gtggccaccg tggccgagaa aacaaaagaa caagtgacca acgtgggcgg
      601 agccgtcgtg acaggcgtga cagctgtggc tcagaaaacc gtggaaggcg ccggatctat
      661 cgccgctgcc accggctttg tgaagaagga ccagctgggc aagaacgagg aaggcgctcc
      721 ccaggaaggc atcctggaag atatgcccgt ggaccccgac aacgaggcct acgagatgcc
      781 tagcgaagag ggctaccagg actacgagcc cgaggcctaa gcggccgcgg tacctggagc
      841 acaagactgg cctcatgggc cttccgctca ctgcccgctt tccagtcggg aaacctgtcg
      901 tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct ctccgcttcc
      961 tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc taatgagcaa
     1021 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1081 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1141 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1201 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1261 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1321 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1381 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1441 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1501 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1561 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1621 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1681 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1741 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1801 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1861 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1921 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gaaccacgct
     1981 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2041 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2101 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2161 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2221 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2281 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2341 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2401 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2461 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2521 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2581 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2641 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2701 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2761 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
