LOCUS       sequence-78433-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHSN6I01
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct aagctcacgt
       61 gacggaatta agcttcgact tgccttccgc acaatacatc atttcttctt agcttttttt
      121 cttcttcttc gttcatacag tttttttttg tttatcagct tacattttct tgaaccgtag
      181 ctttcgtttt cttcttttta actttccatt cggagttttt gtatcttgtt tcatagtttg
      241 tcccaggatt agaatgatta ggcatcgaac cttcaagaat ttgattgaat aaaacatctt
      301 cattcttaag atatgaagat aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc
      361 aggcccattt atatgggaaa gaacaatagt atttcttata taggcccatt taagttgaaa
      421 acaatcttca aaagtcccac atcgcttaga taagaaaacg aagctgagtt tatatacagc
      481 tagagtcgaa gtagtgattg ggagaccaac ccagtggaca taagcctgtt cggttcgtaa
      541 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
      601 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
      661 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
      721 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
      781 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
      841 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
      901 cctgaagcca cacagtgata ttgatttgct
//
