LOCUS       sequence-78435-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN501
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctaagct
       61 cacgtgacgg aattaagctt cgacttgcct tccgcacaat acatcatttc ttcttagctt
      121 tttttcttct tcttcgttca tacagttttt ttttgtttat cagcttacat tttcttgaac
      181 cgtagctttc gttttcttct ttttaacttt ccattcggag tttttgtatc ttgtttcata
      241 gtttgtccca ggattagaat gattaggcat cgaaccttca agaatttgat tgaataaaac
      301 atcttcattc ttaagatatg aagataatct tcaaaaggcc cctgggaatc tgaaagaaga
      361 gaagcaggcc catttatatg ggaaagaaca atagtatttc ttatataggc ccatttaagt
      421 tgaaaacaat cttcaaaagt cccacatcgc ttagataaga aaacgaagct gagtttatat
      481 acagctagag tcgaagtagt gattgggaga ccaacccagt ggacataagc ctgttcggtt
      541 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      601 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
      661 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
      721 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
      781 atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
      841 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
      901 ggcggcctga agccacacag tgatattgat ttgctggtta c
//
