LOCUS       sequence-78436-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN411
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        49..428
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
ORIGIN
        1 tccaggtctc caagttctag gattttcaga actgcaactt attttatcaa ggaatcttta
       61 aacatacgaa cagatcactt aaagttcttc tgaagcaact taaagttatc aggcatgcat
      121 ggatcttgga ggaatcagat gtgcagtcag ggaccatagc acaagacagg cgtcttctac
      181 tggtgctacc agcaaatgct ggaagccggg aacactgggt acgttggaaa ccacgtgatg
      241 tgaagaagta agataaactg taggagaaaa gcatttcgta gtgggccatg aagcctttca
      301 ggacatgtat tgcagtatgg gccggcccat tacgcaattg gacgacaaca aagactagta
      361 ttagtaccac ctcggctatc cacatagatc aaagctgatt taaaagagtt gtgcagatga
      421 tccgtggcgt gagaccaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
      481 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      541 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
      601 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
      661 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg
      721 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
      781 aaccgacgtt gctggccgta catttgtacg gctc
//
