LOCUS       sequence-78437-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN6I11
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        16..395
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
ORIGIN
        1 gcaacttatt ttatcaagga atctttaaac atacgaacag atcacttaaa gttcttctga
       61 agcaacttaa agttatcagg catgcatgga tcttggagga atcagatgtg cagtcaggga
      121 ccatagcaca agacaggcgt cttctactgg tgctaccagc aaatgctgga agccgggaac
      181 actgggtacg ttggaaacca cgtgatgtga agaagtaaga taaactgtag gagaaaagca
      241 tttcgtagtg ggccatgaag cctttcagga catgtattgc agtatgggcc ggcccattac
      301 gcaattggac gacaacaaag actagtatta gtaccacctc ggctatccac atagatcaaa
      361 gctgatttaa aagagttgtg cagatgatcc gtggcgtgag accaacccag tggacataag
      421 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
      481 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
      541 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
      601 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
      661 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
      721 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
      781 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
      841 ta
//
