LOCUS       sequence-78439-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 
            promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUN6A11
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..473
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
ORIGIN
        1 aagctcaagc taagctcacg tgacggaatt aagcttagta attcatccag gtctccaagt
       61 tctaggattt tcagaactgc aacttatttt atcaaggaat ctttaaacat acgaacagat
      121 cacttaaagt tcttctgaag caacttaaag ttatcaggca tgcatggatc ttggaggaat
      181 cagatgtgca gtcagggacc atagcacaag acaggcgtct tctactggtg ctaccagcaa
      241 atgctggaag ccgggaacac tgggtacgtt ggaaaccacg tgatgtgaag aagtaagata
      301 aactgtagga gaaaagcatt tcgtagtggg ccatgaagcc tttcaggaca tgtattgcag
      361 tatgggccgg cccattacgc aattggacga caacaaagac tagtattagt accacctcgg
      421 ctatccacat agatcaaagc tgatttaaaa gagttgtgca gatgatccgt ggcgtgagac
      481 caacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
      541 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      601 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
      661 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
      721 agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc gccgaagtat
      781 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
      841 ccgtacattt gtacggctcc gcagtg
//
