LOCUS       sequence-78446-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal 
            domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..46
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagatgg cccccaagaa
       61 gaagaggaag gtgggcattc accgcggggt acctatggtg gacttgagga cactcggtta
      121 ttcgcaacag caacaggaga aaatcaagcc taaggtcagg agcaccgtcg cgcaacacca
      181 cgaggcgctt gtggggcatg gcttcactca tgcgcatatt gtcgcgcttt cacagcaccc
      241 tgcggcgctt gggacggtgg ctgtcaaata ccaagatatg attgcggccc tgcccgaagc
      301 cacgcacgag gcaattgtag gggtcggtaa acagtggtcg ggagcgcgag cacttgaggc
      361 gctgctgact gtggcgggtg agcttagggg gcctccgctc cagctcgaca ccgggcagct
      421 gctgaagatc gcgaagagag ggggagtaac agcggtagag gcagtgcacg cctggcgcaa
      481 tgcgctcacc ggggccccct tgaacctgac cccagaccag gtagtcgcaa tcgcgtcaaa
      541 cggaggggga aagcaagccc tggaaaccgt gcaaaggttg ttgccggtcc tttgtcaaga
      601 ccacggcctt acaccggagc aagtcgtggc cattgcatcc cacgacggtg gcaaacaggc
      661 tcttgagacg gttcagagac ttctcccagt tctctgtcaa gcccacgggc tgactcccga
      721 tcaagttgta gcgattgcgt cnaacattgg agggaaacaa gcattggaga ctgtccaacg
      781 gctccttccc gtgttgtgtc aagcccacgg nttgacgcct gcacaagtgg
//
