LOCUS       sequence-78449-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal 
            domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..633)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 accgaggaga gggttaggga taggcttacc ttcgaagggc ccttaaaagt ttatctcgcc
       61 gttattaaat ttccgtctca cttcctctaa ggttaatgtg ccggctttaa tcatttctcc
      121 accaattaaa agctcttcta cactaagaac agctccatta caattagtga tatgatttaa
      181 tcgtgtaagc tgagctttgt agtttccttt aaagtgacca ctcacaaata aaaacttaaa
      241 ttccgttaca gaagatggat agactttcca ccattcatta gggttgagat gtttgtttcg
      301 tgtttgattt tcttcgacat atcgctccat ttcatctgct tggccaattg gcagattata
      361 acctccgcta taagctttag tatccacgat cacaccgtaa tcaataggag atccgacagt
      421 ataaattgct ccgtccggtt tccttgatcc acccaaatgt ttacctctat atccataaac
      481 tttcataaaa aattccatta ccttcatttc aagaattcta tcctgagtgg aatttctggc
      541 aatttcaatt aattcaatat attcatgagg cacatatttc aatttatgac gaagttcaga
      601 tttcttctcc tccagttcac ttttgactag ttgggatccc gcgacttgat gggaagttct
      661 ctcgggaatc cgttggttgg ttctttggat caatgcggga gcatgaggca gacccttttt
      721 gactgcatcg agcgcgggtc gtccaccaag acatgccaac gccaccagat ggtcattcgt
      781 taacgcagcc aacgcgggat cgggcctgga caattgggct acgatggact
//
