LOCUS       sequence-78454-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with Q7 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-CtermQ7
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..632)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 ccgaggagag ggttagggat aggcttacct tcgaagggcc cttaaaagtt tatctcgccg
       61 ttattaaatt tccgtctcac ttcctctaag gttaatgtgc cggctttaat catttctcca
      121 ccaattaaaa gctcttctac actaagaaca gctccattac aattagtgat atgatttaat
      181 cgtgtaagct gagctttgta gtttccttta aagtgaccac tcacaaataa aaacttaaat
      241 tccgttacag aagatggata gactttccac cattcattag ggttgagatg tttgtttcgt
      301 gtttgatttt cttcgacata tcgctccatt tcatctgctt ggccaattgg cagattataa
      361 cctccgctat aagctttagt atccacgatc acaccgtaat caataggaga tccgacagta
      421 taaattgctc cgtccggttt ccttgatcca cccaaatgtt tacctctata tccataaact
      481 ttcataaaaa attccattac cttcatttca agaattctat cctgagtgga atttctggca
      541 atttcaatta attcaatata ttcatgaggc acatatttca atttatgacg aagttcagat
      601 ttcttctcct ccagttcact tttgactagt tgggatcccg cgacttgatg ggaagttctc
      661 tcgggaattt gttggttggt ttgttggatc aatgcgggag catgaggcag accttgttgg
      721 actgcatcga gcgcgggtcg tccaccaaga catgccaacg ccaccagatg gtcattcgtt
      781 aacgcagcca acgcgggatc gggcctggac aattgggcta cgatggactc
//
