LOCUS       sequence-78456-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..66
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             73..93
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caggactaaa gacatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat
       61 gacaagatgg cccccaagaa gaagaggaag gtgggcattc accgcggggt acctatggtg
      121 gacttgagga cactcggtta ttcgcaacag caacaggaga aaatcaagcc taaggtcagg
      181 agcaccgtcg cgcaacacca cgaggcgctt gtggggcatg gcttcactca tgcgcatatt
      241 gtcgcgcttt cacagcaccc tgcggcgctt gggacggtgg ctgtcaaata ccaagatatg
      301 attgcggccc tgcccgaagc cacgcacgag gcaattgtag gggtcggtaa acagtggtcg
      361 ggagcgcgag cacttgaggc gctgctgact gtggcgggtg agcttagggg gcctccgctc
      421 cagctcgaca ccgggcagct gctgcagatc gcgaagagag ggggagtaac agcggtagag
      481 gcagtgcacg cctggcgcaa tgcgctcacc ggggccccct tgaacctgac cccagaccag
      541 gtagtcgcaa tcgcgtcaaa cggaggggga aagcaagccc tggaaaccgt gcaaaggttg
      601 ttgccggtcc tttgtcaaga ccacggcctt acaccggagc aagtcgtggc cattgcatcc
      661 cacgacggtg gcaaacaggc tcttgagacg gttcagagac ttctcccagt tctctgtcaa
      721 gcccacgggc tgactcccga tcaagttgta gcgattgcgt cgaacattgg agggaaacaa
      781 gcattggaga ctgtccaacg gctccctccc gtgttgtgtc aagcccacgg tttgacgcct
      841 gcacaagtgg
//
