LOCUS       sequence-78461-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN2
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..41
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             48..68
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ataaagatca tgacatcgat tacaaggatg acgatgacaa gatggccccc aagaagaaga
       61 ggaaggtggg cattcaccgc ggggtaccta tggtggactt gaggacactc ggttattcgc
      121 aacagcaaca ggagaaaatc aagcctaagg tcaggagcac cgtcgcgcaa caccacgagg
      181 cgcttgtggg gcatggcttc actcatgcgc atattgtcgc gctttcacag caccctgcgg
      241 cgcttgggac ggtggctgtc aaataccaag atatgattgc ggccctgccc gaagccacgc
      301 acgaggcaat tgtaggggtc ggtaaacagt ggtcgggagc gcgagcactt gaggcgctgc
      361 tgactgtggc gggtgagctt agggggcctc cgctccagct cgacaccggg cagctgctgc
      421 agatcgcgca gagaggggga gtaacagcgg tagaggcagt gcacgcctgg cgcaatgcgc
      481 tcaccggggc ccccttgaac ctgaccccag accaggtagt cgcaatcgcg tcaaacggag
      541 ggggaaagca agccctggaa accgtgcaaa ggttgttgcc ggtcctttgt caagaccacg
      601 gccttacacc ggagcaagtc gtggccattg catcccacga cggtggcaaa caggctcttg
      661 agacggttca gagacttctc ccagttctct gtcaagccca cgggctgact cccgatcaag
      721 ttgtagcgat tgcgtcgaac attggaggga aacaagcatt ggagactgtc ccacggctct
      781 ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca agtggtcgcc
//
