LOCUS       sequence-78464-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N2 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN2
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(53..640)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 aaatcgagac gaggagaggg ttagggatag gcttaccttc gaagggccct taaaagttta
       61 tctcgccgtt attaaatttc cgtctcactt cctctaaggt taatgtgccg gctttaatca
      121 tttctccacc aattaaaagc tcttctacac taagaacagc tccattacaa ttagtgatat
      181 gatttaatcg tgtaagctga gctttgtagt ttcctttaaa gtgaccactc acaaataaaa
      241 acttaaattc cgttacagaa gatggataga ctttccacca ttcattaggg ttgagatgtt
      301 tgtttcgtgt ttgattttct tcgacatatc gctccatttc atctgcttgg ccaattggca
      361 gattataacc tccgctataa gctttagtat ccacgatcac accgtaatca ataggagatc
      421 cgacagtata aattgctccg tccggtttcc ttgatccacc caaatgttta cctctatatc
      481 cataaacttt cataaaaaat tccattacct tcatttcaag aattctatcc tgagtggaat
      541 ttctggcaat ttcaattaat tcaatatatt catgaggcac atatttcaat ttatgacgaa
      601 gttcagattt cttctcctcc agttcacttt tgactagttg ggatcccgcg actcgatggg
      661 aagttctctc gggaatccgc cggttggttc ttttgatcaa tgcgggagca tgangcagaa
      721 cctttttgac tgcatcgagc gcgggtcgtc caccaagaca tggcaacgcc cacagatggt
      781 cattcgttaa cgcagccaac gcgggatcgg gcctggacaa ttgggctacg
//
