LOCUS       sequence-78469-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5L18-NtermN3
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..609)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRNKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 cttaccttcg aagggccctt aaaagtttat ctcgccgtta ttaaatttcc gtctcacttc
       61 ctctaaggtt aatgtgccgg ctttaatcat ttctccacca attaaaagct cttctacact
      121 aagaacagct ccattacaat tagtgatatg atttaatcgt gtaagctgag ctttgtagtt
      181 tcctttaaag tgaccactca caaataaaaa cttaaattcc gttacagaag atggatagac
      241 tttccaccat tcattagggt tgagatgttt gtttcgtgtt tgattttctt cgacatatcg
      301 ctccatttca tctgcttggc caattggcag attataacct ccgctataag ctttagtatc
      361 cacgatcaca ccgtaatcaa taggagatcc gacagtataa attgctccgt ccggtttcct
      421 tgatccaccc aaatgtttac ctctatatcc ataaactttc ataaaaaatt ccattacctt
      481 catttcaaga attctatcct gagtggaatt tctggcaatt tcaattaatt caatatattc
      541 atgaggcaca tatttcaatt tatgacgaag ttcagatttc ttctcctcca gttcactttt
      601 gactagttgg gatcccgcga ctcgatggga agttctctcg ggaatccgcc ggttggttct
      661 tttgatcaat gcgggagcat gaggcagacc ctttttgact gcatcgagcg cgggtcgtcc
      721 accaagacat gccaacgcca ccagatggtc attcgttaac gcagccaacg cgggatccgg
      781 cctggacaat tgggctacga tggactccaa
//
