LOCUS       sequence-78474-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with canonical 
            C-terminal domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-CtermCan
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..631)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 cgaggagagg gttagggata ggcttacctt cgaagggccc ttaaaagttt atctcgccgt
       61 tattaaattt ccgtctcact tcctctaagg ttaatgtgcc ggctttaatc atttctccac
      121 caattaaaag ctcttctaca ctaagaacag ctccattaca attagtctta tgatttaatc
      181 gtgtaagctg agctttgtag tttcctttaa agtgaccact cacaaataaa aacttaaatt
      241 ccgttacaga agatggatag actttccacc attcattagg gttgatatgt ttgtttcgtg
      301 tttgattttc tttgacatat cgttgcattt catctgcttg gccaattggc agattataac
      361 ctccgctata agctttagta tccacgatca caccgtaatc aataggagat ccgacagtat
      421 aaattgctcc gtccggtttc cttgatccac ccaaatgttt acctctatat ccataaactt
      481 tcataaaaaa ttccattacc ttcatttcaa gaattctatc ctgagtggaa tttctggcaa
      541 tttcaattaa ttcaatatat tcatgaggca catatttcaa tttatgacga agttcagatt
      601 tcttctcctc cagttcactt ttgactagtt gggatcccgc gactcgatgg gaagttctct
      661 cgggaatccg ccggttggtt cttttgatca atgcgggagc atgaggcaga ccctttttga
      721 ctgcatcgag cgcgggtcgt ccaccaagac atgccaacgc caccagatgg tcattcgtta
      781 acgcagccaa cgcgggatcg ggcctggaca attgggctac gatggactcc
//
