LOCUS       sequence-78476-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-CtermQ3
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             56..76
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga tggcccccaa
       61 gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg gtggacttga ggacactcgg
      121 ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc aggagcaccg tcgcgcaaca
      181 ccacgaggcg cttgtggggc atggcttcac tcatgcgcat attgtcgcgc tttcacagca
      241 ccctgcggcg cttgggacgg tggctgtcaa ataccaagat atgattgcgg ccctgcccga
      301 agccacgcac gaggcaattg taggggtcgg taaacagtgg tcgggagcgc gagcacttga
      361 ggcgctgctg actgtggcgg gtgagcttag ggggcctccg ctccagctcg acaccgggca
      421 gctgctgaag atcgcgaaga gagggggagt aacagcggta gaggcagtgc acgcctggcg
      481 caatgcgctc accggggccc ccttgaacct gaccccagac caggtagtcg caatcgcgtc
      541 acatgacggg ggaaagcaag ccctgnaaac cgtgcaaagg ttgttgccgg tcctttgtca
      601 agaccacggc cttacaccgg agcaagtcgt ggccattgca agcaatgggg gtggcaaaca
      661 ggctcttgan acggttcaga gacttctccc agttctctgt caagcccacn ggctgactcc
      721 cgatcaagtt gtagcgattg cgtccaacgg tgganggaaa caagcatnnn agactgtcca
      781 acggctcttc ccgtgntgtg tcnagncnnc gnnttgacgc ctgnncaag
//
