LOCUS       sequence-78486-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..69
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agacaggact aaagacatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg
      121 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc
      181 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat
      241 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat
      301 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg
      361 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg
      421 ctccagctcg acaccgggca gctgctgcag atcgcgaaga gagggggagt aacagcggta
      481 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gaccccagac
      541 caggtagtcg caatcgcgtc acatgacggg ggaaagcaag ccctggaaac cgtgcaaagg
      601 ttgttgccgg tcctttgtca agaccacggc cttacaccgg agcaagtcgt ggccattgca
      661 agcaatgggg gtggcaaaca ggctcttgag acggttcaga gacttctccc agttctctgt
      721 caagcccacg ggctgactcc cgatcaagtt gtagcgattg cgtccaacgg tggagggaaa
      781 caagcattgg agactgtcca acggctcctt cccgtgttgt gtcaagccca cggtttgacg
      841 cctgcacaag
//
