LOCUS       sequence-78489-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..658)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 aggagagacg gtaccgtaaa tcgagaccga ggagagggtt agggataggc ttaccttcga
       61 agggccctta aaagtttatc tcgccgttat taaatttccg tctcacttcc tctaaggtta
      121 atgtgccggc tttaatcatt tctccaccaa ttaaaagctc ttctacacta agaacagctc
      181 cattacaatt agtcttatga tttaatcgtg taagctgagc tttgtagttt cctttaaagt
      241 gaccactcac aaataaaaac ttaaattccg ttacagaaga tggatagact ttccaccatt
      301 cattagggtt gatatgtttg tttcgtgttt gattttcttt gacatatcgt tgcatttcat
      361 ctgcttggcc aattggcaga ttataacctc cgctataagc tttagtatcc acgatcacac
      421 cgtaatcaat aggagatccg acagtataaa ttgctccgtc cggtttcctt gatccaccca
      481 aatgtttacc tctatatcca taaactttca taaaaaattc cattaccttc atttcaagaa
      541 ttctatcctg agtggaattt ctggcaattt caattaattc aatatattca tgaggcacat
      601 atttcaattt atgacgaagt tcagatttct tctcctccag ttcacttttg actagttggg
      661 atcccgcgac tcgatgggaa gttctctcgg gaatccgccg gttggttctt ttgatcaatg
      721 cgggagcatg aggcagaccc tttttgactg catcgagcgc gggtcgtcca ccaagacatg
      781 ccaacgccac cagatggtca ttcgttaacg cagccaacgc gggatcgggg ctggacaatt
      841 gggctacgat
//
