LOCUS       sequence-78491-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..61
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             68..88
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacaagacat gacggtgatt ataaagatca tgacatcgat tacaaggatg acgatgacaa
       61 gatggccccc aagaagaaga ggaaggtggg cattcaccgc ggggtaccta tggtggactt
      121 gaggacactc ggttattcgc aacagcaaca ggagaaaatc aagcctaagg tcaggagcac
      181 cgtcgcgcaa caccacgagg cgcttgtggg gcatggcttc actcatgcgc atattgtcgc
      241 gctttcacag caccctgcgg cgcttgggac ggtggctgtc aaataccaag atatgattgc
      301 ggccctgccc gaagccacgc acgaggcaat tgtaggggtc ggtaaacagt ggtcgggagc
      361 gcgagcactt gaggcgctgc tgactgtggc gggtgagctt agggggcctc cgctccagct
      421 cgacaccggg cagctgctgc agatcgcgca gagaggggga gtaacagcgg tagaggcagt
      481 gcacgcctgg cgcaatgcgc tcaccggggc ccccttgaac ctgaccccag accaggtagt
      541 cgcaatcgcg tcacatgacg ggggaaagca agccctggaa accgtgcaaa ggttgttgcc
      601 ggtcctttgt caagaccacg gccttacacc ggagcaagtc gtggccattg caagcaatgg
      661 gggtggcaaa caggctcttg agacggttca gagacttctc ccagttctct gtcaagccca
      721 cgggctgact cccgatcaag ttgtagcgat tgcgtccaac ggtggaggga aaacagcatt
      781 ggagactgtc caacggctcc ttcccgtgtt gtgtcaagcc cacggtttga cgcctgcaca
      841 agtggtcgcc
//
