LOCUS       sequence-78494-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N2 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..661)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gcacggagag acggtaccgt aaatcgagac cgaggagagg gttagggata ggcttacctt
       61 cgaagggccc ttaaaagttt atctcgccgt tattaaattt ccgtctcact tcctctaagg
      121 ttaatgtgcc ggctttaatc atttctccac caattaaaag ctcttctaca ctaagaacag
      181 ctccattaca attagtctta tgatttaatc gtgtaagctg agctttgtag tttcctttaa
      241 agtgaccact cacaaataaa aacttaaatt ccgttacaga agatggatag actttccacc
      301 attcattagg gttgatatgt ttgtttcgtg tttgattttc tttgacatat cgttgcattt
      361 catctgcttg gccaattggc agattataac ctccgctata agctttagta tccacgatca
      421 caccgtaatc aataggagat ccgacagtat aaattgctcc gtccggtttc cttgatccac
      481 ccaaatgttt acctctatat ccataaactt tcataaaaaa ttccattacc ttcatttcaa
      541 gaattctatc ctgagtggaa tttctggcaa tttcaattaa ttcaatatat tcatgaggca
      601 catatttcaa tttatgacga agttcagatt tcttctcctc cagttcactt ttgactagtt
      661 gggatcccgc gactcgatgg gaagttctct cgggaatccg ccggttggtt cttttgatca
      721 atgcgggagc atgaggcaga ccctttttga ctgcatcgag cgcgggtcgt ccaccaagac
      781 atgccaacgc caccagatgg tcattcgtta acgcagccaa cgcgggatcg ggcctggaca
      841 attgggctac
//
