LOCUS       sequence-78496-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal 
            domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Guilinger JP, Pattanayak V, Reyon D, Tsai SQ, Sander JD, Joung JK, 
            Liu DR
  TITLE     Broad specificity profiling of TALENs results in engineered 
            nucleases with improved DNA-cleavage specificity.
  JOURNAL   Nat Methods. 2014 Apr;11(4):429-35. doi: 10.1038/nmeth.2845. Epub 
            2014 Feb 16.
  PUBMED    24531420
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2845"; journalName: "Nat Methods"; date: "2014-04"; 
            volume: "11"; issue: "4"; pages: "429-35"
COMMENT     Sequence Label: TALEN-CCR5R18-NtermN3
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..60
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             67..87
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taaagacatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
       61 atggccccca agaagaagag gaaggtgggc attcaccgcg gggtacctat ggtggacttg
      121 aggacactcg gttattcgca acagcaacag gagaaaatca agcctaaggt caggagcacc
      181 gtcgcgcaac accacgaggc gcttgtgggg catggcttca ctcatgcgca tattgtcgcg
      241 ctttcacagc accctgcggc gcttgggacg gtggctgtca aataccaaga tatgattgcg
      301 gccctgcccg aagccacgca cgaggcaatt gtaggggtcg gtaaacagtg gtcgggagcg
      361 cgagcacttg aggcgctgct gactgtggcg ggtgagctta gggggcctcc gctccagctc
      421 gacaccgggc agctgctgca gatcgcgcag caggggggag taacagcggt agaggcagtg
      481 cacgcctggc gcaatgcgct caccggggcc cccttgaacc tgaccccaga ccaggtagtc
      541 gcaatcgcgt cacatgacgg gggaaagcaa gccctggaaa ccgtgcaaag gttgttgccg
      601 gtcctttgtc aagaccacgg ccttacaccg gagcaagtcg tggccattgc aagcaatggg
      661 ggtggcaaac aggctcttga gacggttcag agacttctcc cagttctctg tcaagcccac
      721 gggctgactc ccgatcaagt tgtagcgatt gcgtccaacg gtggagggaa acaagcattg
      781 gagactgtcc aacggctcct tcccgtgttg tgtcaagccc acggtttgac gcctgcacaa
      841 gtggtcgcca
//
