LOCUS       sequence-78508-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Tamura M, Gu J, Danen EH, Takino T, Miyamoto S, Yamada KM
  TITLE     PTEN interactions with focal adhesion kinase and suppression of the 
            extracellular matrix-dependent phosphatidylinositol 3-kinase/Akt 
            cell survival pathway.
  JOURNAL   J Biol Chem. 1999 Jul 16;274(29):20693-703.
  PUBMED    10400703
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1999-07-16"; volume: "274(29)"; pages: "20693-703"
COMMENT     Sequence Label: pCDNA HA FAK Y577F
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacca tgtacgacgt tcctgattac
      121 gctagcctcc cgagatccgg tatggcagct gcttatcttg acccaaactt gaatcacaca
      181 ccaagttcga gtactaagac tcacctgggt actggcatgg aacggtcccc tggtgcaatg
      241 gaacgagtat taaaggtctt tcatcatttt gaaagcagta gtgagccaac cacctgggcc
      301 agtattatca ggcatggaga tgctactgat gtcaggggca tcattcagaa gatagtggac
      361 agtcacaaag taaagcacgt ggcctgctat ggatttcgcc tcagtcacct gcgatcggag
      421 gaggtgcact ggctgcacgt ggacatgggt gtctccagtg tgagggagaa atatgaactc
      481 gcccacccac cagaggagtg gaaatatgag ttgagaattc gttacttgcc aaaaggattt
      541 ctaaaccagt ttactgaaga taagccaaca ttgaatttct tctatcaaca ggtgaagagt
      601 gactacatgc aagaaatagc tgatcaagta gaccaagaaa tagctttgaa gttgggttgt
      661 ttggaaatta ggcgatccta ttgggagatg aggggtaatg ccctagagaa gaagtccaac
      721 tatgaagtat tagaaaaaga tgttggttta aagcgatttt ttcctaagag tttactggat
      781 tctgtcaagg ccaaaacact aagaaagctg atccagc
//
