LOCUS       sequence-78515-e        6020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses photoconvertible dendra in macrophages.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Harvie EA, Green JM, Neely MN, Huttenlocher A
  TITLE     Innate immune response to Streptococcus iniae infection in zebrafish
            larvae.
  JOURNAL   Infect Immun. 2013 Jan;81(1):110-21. doi: 10.1128/IAI.00642-12. Epub
            2012 Oct 22.
  PUBMED    23090960
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/IAI.00642-12"; journalName: "Infect Immun"; date: 
            "2013-01"; volume: "81"; issue: "1"; pages: "110-21"
COMMENT     Sequence Label: Tol2-mpeg1-dendra2
FEATURES             Location/Qualifiers
     source          1..6020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(561..572,573..710)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             2610..3302
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     polyA_signal    3424..3545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3461..3480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3515..3534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(3582..3769,3770..3781)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3836..3856)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3836..3854)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3875..3891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3875..3891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3888..3910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3899..3915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3923..3929,3930..3947,3948..3953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3968..3989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4106..4123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4277..4865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4357..4376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5036..5827,5828..5896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5659..5678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5897..6001)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aaggctagca agatctgctc gagtgttgga gcacatctga catctgaaat tatttgaaag
      781 cagcagaaat gtttttacag ctattacaaa atttacttaa caagaatcct ttaaaatatg
      841 acaatttatt gccaataatt tacagtaagt tttgcagtac tttaatatta caaattatac
      901 aactgtgctg gcacacaaaa aataacttaa tccagcacaa caacaacaac aacaactaca
      961 aaaagcatat ttccttttgc tttttcttgt tcactcaact tttggaaaca tcaactgcac
     1021 tgacctaaaa ttatacagta tgtcagtaat acaaaaacac ttagttaggt taacataaga
     1081 ttattagttt tctggagagg tgaactactc tgtaagaaac ctttttaagt tgtgccaact
     1141 aaacattttt atcagcagaa ctacaatgct tgatgagtga acaaaaaaac aaacaaacct
     1201 tatttccttt tgttttttct tgtgtactta acattttttt cacctttatt tgaaacgaca
     1261 gtagggagta tggacaggaa agtgttggga gaggagaggg atgagcatag aaccaggagg
     1321 taggaatcaa actcaggtca ctatgaacac cggagtgcca ggtgtcaaca cactaaccac
     1381 tacaccatca actccaacat ttacttgaaa acatcagttg agtgaacaaa aaactcttgt
     1441 ggaaattggt actgagaaat aatacattat cacaacaaac aaaacagaga caaatgttaa
     1501 caaaacaatt attagaaaca gaattagaaa ccggacagga ggtaaaacaa taatgataaa
     1561 caagacaagg atcaaaacat ggcaggacaa agacagtttt ttgttcactc aacctcagat
     1621 attttaggtc agtgcaactg atgtttacaa aggttgtatg catgtcaaca ccaatggtgt
     1681 agtgattagt gtgttgacac aaggcactcc agtgttcaca ctgatgctgg tttgattccc
     1741 acatcgatgt cctttgctaa tcctgcctct ctccccatgc tttcctgtct atactctcta
     1801 ctgttgtgtc aattaaaggt gaaaaaagtt aagcacaaaa gaaaaatcta aggatataag
     1861 gattatgttt ttttttgttc actcaaaatc aggcattcca gttctgcaga caaaaaatgt
     1921 tcagttggca caacttaaaa agttttgtta cagagtagtt cacctctcca gaaaactaat
     1981 aatcttatgt taacccaact aaatgttttt actgaaaaat aattgaatgt cagtgcagct
     2041 gatgtttttt taaaagtgga gagaacaaaa aaagtgaaag gaaataagga ttcattgttg
     2101 taccgactta tttttttgaa gaaaattctg ctatagggct ttcatgatcc cctatgctca
     2161 cagcagtttt aggagatgct tataataaag aggatttgaa aaacctgcta atcttgtaca
     2221 tttctaaaac aagaagccaa ccaatttcaa ttcctcctct ttcaaagcca atggaaaatt
     2281 taaaacgcac agtctcttgc gtcatcaaaa ccattctgaa gactcatcaa aatccttcgt
     2341 agctttaaat ataaatctat tattgaacag atcatacttc acaaaaaata taaatatatt
     2401 tgattcattc acttcatgaa attgtccttc aacttaacgt ttgaacgcaa ggtaccaaaa
     2461 cacaaagagg aacctcatag tctcatgacc cttcccccac ggatttgaaa ctgagaagtg
     2521 gttttattga ggaactcagc aaaatctata atcaccatat ttgattgaaa atcacattag
     2581 tgcaggagac agcaaaagga tccgccacca tgaacacccc gggaattaac ctgatcaagg
     2641 aggacatgcg cgtgaaggtg cacatggagg gcaacgtgaa cggccacgcc ttcgtgatcg
     2701 agggcgaggg caagggcaag ccctacgagg gcacccagac cgccaacctg accgtgaagg
     2761 agggcgcccc cctgcccttc agctacgaca tcctgaccac cgccgtgcac tacggcaacc
     2821 gggtgttcac caagtacccc gaggacatcc ccgactactt caagcagagc ttccccgagg
     2881 gctacagctg ggagcgcacc atgaccttcg aggacaaggg catctgcacc atccgcagcg
     2941 acatcagcct ggagggcgac tgcttcttcc agaacgtgcg cttcaagggc accaacttcc
     3001 cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc accgagaagc
     3061 tgcacgtgcg cgacggcctg ctggtgggca acatcaacat ggccctgctg ctggagggcg
     3121 gcggccacta cctgtgcgac ttcaagacca cctacaaggc caagaaggtg gtgcagctgc
     3181 ccgacgccca cttcgtggac caccgcatcg agatcctggg caacgacagc gactacaaca
     3241 aggtgaagct gtacgagcac gccgtggccc gctacagccc cctgcccagc caggtgtggt
     3301 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     3361 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     3421 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     3481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     3541 tcttaaggcg agatcctgcg gccgcaaggg aaaatagaat gaagtgatct ccaaaaaata
     3601 agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa
     3661 atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc
     3721 cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct
     3781 ggttcttgac cccctacctt cagcaagccc agcagatcca ctagatcttt tgttcccttt
     3841 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     3901 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3961 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4021 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4081 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4681 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     5881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     5941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6001 gcacatttcc ccgaaaagtg
//
