LOCUS       sequence-78517-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid compatible with the Golden Gate TALEN cloning system. To 
            clone TAL effectors 6.5 RVDs long. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pFUSC6
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(26..46)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(26..44)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(65..81)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(65..81)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(78..100)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    89..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(113..119,120..137,138..143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    158..179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cctcgaggtc ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
       61 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      181 ttgcgttgcg ctcactgccc gctttccacc ggtggtctca cggtgcagcg gctgttgccg
      241 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcga gacgtctaga
      301 ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac
      361 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
      421 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      481 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
      541 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
      601 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg
      661 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
      721 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
      781 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
      841 ggcgagcttt gatcaacgac cttttggaaa cttc
//
