LOCUS       sequence-78523-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid compatible with the Golden Gate TALEN cloning system. To 
            clone TAL effectors 8.5 RVDs long..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pFUSC8
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(28..48)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(28..46)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(67..83)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(67..83)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(80..102)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    91..107
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(115..121,122..139,140..145))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    160..181
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cccctcgagg tcctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
       61 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      121 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      181 aattgcgttg cgctcactgc ccgctttcca ccggtggtct cctcgaaacg gtgcagcggc
      241 tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg gctatcgaga
      301 cgtctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg
      361 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
      421 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      481 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
      541 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
      601 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
      661 tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
      721 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
      781 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
      841 aacaacgcgg cgagctttga tcaacgacct tttggaaact tc
//
