LOCUS       sequence-78525-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid compatible with the Golden Gate TALEN cloning system. To 
            clone TAL effectors 9.5 RVDs long..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Boissel S, Jarjour J, Astrakhan A, Adey A, Gouble A, Duchateau P, 
            Shendure J, Stoddard BL, Certo MT, Baker D, Scharenberg AM
  TITLE     megaTALs: a rare-cleaving nuclease architecture for therapeutic 
            genome engineering.
  JOURNAL   Nucleic Acids Res. 2013 Nov 26.
  PUBMED    24285304
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Nov 26."
COMMENT     Sequence Label: pFUSC9
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..47)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(27..45)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(66..82)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(66..82)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(79..101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    90..106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(114..120,121..138,139..144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
       61 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      121 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      181 attgcgttgc gctcactgcc cgctttccac cggtggtctc cgctcgaaac ggtgcagcgg
      241 ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgag
      301 acgtctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
      361 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
      421 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
      481 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
      541 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
      601 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
      661 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
      721 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
      781 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
      841 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
      901 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgac
//
