LOCUS       sequence-78529-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pRKVSV-PTEN C124A
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        112..130
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     112..129
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     143..162
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      208..217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             220..252
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             253..285
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
       61 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
      121 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
      181 ctgcacctcg gttctatcga ttcagccgcc accatgggat acaccgacat cgagatgaac
      241 cggctgggca agtacaccga catcgagatg aaccggctgg gcaagggatc caagcttatg
      301 acagccatca tcaaagagat cgttagcaga aacaaaagga gatatcaaga ggatggattc
      361 gacttagact tgacctatat ttatccaaac attattgcta tgggatttcc tgcagaaaga
      421 cttgaaggcg tatacaggaa caatattgat gatgtagtaa ggtttttgga ttcaaagcat
      481 aaaaaccatt acaagatata caatctttgt gctgaaagac attatgacac cgccaaattt
      541 aattgcagag ttgcacaata tccttttgaa gaccataacc caccacagct agaacttatc
      601 aaaccctttt gtgaagatct tgaccaatgg ctaagtgaag atgacaatca tgttgcagca
      661 attcacgcta aagctggaaa gggacgaact ggtgtaatga tatgtgcata tttattacat
      721 cggggcaaat ttttaaaggc acaagaggcc ctagatttct atggggaagt
//
