LOCUS       sequence-78531-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Yamada KM
  TITLE     Yamada Lab Plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pRKVSV-PTEN G129E
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        235..253
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     235..252
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     266..285
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      331..340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             343..375
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             376..408
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 gagctcgttt agtgaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
       61 tagaagacac cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat
      121 tccccgtgcc aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc
      181 ttcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag
      241 gtgacactat agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc
      301 caactgcacc tcggttctat cgattcagcc gccaccatgg gatacaccga catcgagatg
      361 aaccggctgg gcaagtacac cgacatcgag atgaaccggc tgggcaaggg atccaagctt
      421 atgacagcca tcatcaaaga gatcgttagc agaaacaaaa ggagatatca agaggatgga
      481 ttcgacttag acttgaccta tatttatcca aacattattg ctatgggatt tcctgcagaa
      541 agacttgaag gcgtatacag gaacaatatt gatgatgtag taaggttttt ggattcaaag
      601 cataaaaacc attacaagat atacaatctt tgtgctgaaa gacattatga caccgccaaa
      661 tttaattgca gagttgcaca atatcctttt gaagaccata acccaccaca gctagaactt
      721 atcaaaccct tttgtgaaga tcttgaccaa tggctaagtg aagatgacaa tcatgttgca
      781 gcaattcact gtaaagctgg aaaggaacga actggtgtaa tgatatgtgc atatttatta
      841 catcgg
//
