LOCUS       sequence-78535-e         189 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express full length HA tagged p35sr in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 189)
  AUTHORS   Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston 
            DM
  TITLE     Functional role of p35srj, a novel p300/CBP binding protein, during 
            transactivation by HIF-1.
  JOURNAL   Genes Dev. 1999 Jan 1. 13(1):64-75.
  PUBMED    9887100
REFERENCE   2  (bases 1 to 189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            1999 Jan 1. 13(1):64-75."
COMMENT     Sequence Label: SB51
FEATURES             Location/Qualifiers
     source          1..189
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(38..64)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(44..70)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(44..70)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             69..95
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             69..95
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             75..101
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        complement(132..150)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(133..150)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(171..188)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagtcagag ggaggcgtag tcgggcacgt
       61 cgtaggggta cccctacgac gtgcccgact acgcctccct ctgactcgag catgcatcta
      121 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatgcag cctcgactgt
      181 gccttctaa
//
