LOCUS       sequence-78537-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA
  TITLE     How capping protein binds the barbed end of the actin filament.
  JOURNAL   Curr Biol. 2003 Sep 2. 13(17):1531-7.
  PUBMED    12956956
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Sep 2. 13(17):1531-7."
COMMENT     Sequence Label: pet 3d beta tentacle delete CP
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             102..124
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag
      121 gagatataca tatgagtgac cagcagctgg attgtgcctt ggatttgatg aggcgtctgc
      181 ctccgcagca gatagagaag aatctcagtg acctcattga cttggtccca agcctctgtg
      241 aagatctcct ctcctctgtt gatcagccat tgaagattgc acgagataag gtggtaggaa
      301 aagactatct attgtgtgac tacaacagag atggagactc gtatagatca ccgtggagta
      361 acaagtatga tcctcccctg gaagacggtg ccatgccatc tgctcgcctg cgcaagctgg
      421 aggtggaagc caacaatgcc tttgaccagt atagagactt gtatttcgaa ggtggagtct
      481 cctctgtcta cctctgggat ctggatcatg gttttgccgg tgtgatcctc attaagaaag
      541 ctggagatgg atcaaagaag attaagggat gctgggattc catccacgtg gtggaggttc
      601 aggagaaatc cagcggccgt actgctcatt acaagctgac ctccacagtg atgctgtggc
      661 tgcagactaa taaaactggt tctggtacca tgaaccttgg cgggagcctc accagacaga
      721 tggagaaaga tgagactgtg agcgactctt ctccgcacat agcca
//
