LOCUS       sequence-78538-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA
  TITLE     How capping protein binds the barbed end of the actin filament.
  JOURNAL   Curr Biol. 2003 Sep 2. 13(17):1531-7.
  PUBMED    12956956
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Sep 2. 13(17):1531-7."
COMMENT     Sequence Label: pet 3d alpha tentacle delete CP
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag ggagaccaca acggtttccc tctagaaata attttgttta actttaagaa
      121 ggagatatac atatgagtga ccagcagctg gattgtgcct tggatttgat gaggcgtctg
      181 cctccgcagc agatagagaa gaatctcagt gacctcattg acttggtccc aagcctctgt
      241 gaagatctcc tctcctctgt tgatcagcca ttgaagattg cacgagataa ggtggtagga
      301 aaagactatc tattgtgtga ctacaacaga gatggagact cgtatagatc accgtggagt
      361 aacaagtatg atcctcccct ggaagacggt gccatgccat ctgctcgcct gcgcaagctg
      421 gaggtggaag ccaacaatgc ctttgaccag tatagagact tgtatttcga aggtggagtc
      481 tcctctgtct acctctggga tctggatcat ggttttgccg gtgtgatcct cattaagaaa
      541 gctggagatg gatcaaagaa gattaaggga tgctgggatt ccatccacgt ggtggaggtt
      601 caggagaaat ccagcggccg tactgctcat tacaagctga cctccacagt gatgctgtgg
      661 ctgcagacta ataaaactgg ttctggtacc atgaaccttg gcgggagcct caccagacag
      721 atggagaaag atga
//
