LOCUS       sequence-78539-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Lee BI, Kim KH, Park SJ, Eom SH, Song HK, Suh SW
  TITLE     Ring-shaped architecture of RecR: implications for its role in 
            homologous recombinational DNA repair.
  JOURNAL   EMBO J. 2004 May 19. 23(10):2029-38.
  PUBMED    15116069
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2004 May 19. 23(10):2029-38."
COMMENT     Sequence Label: pET-28b(+)-DR RecR(34-220)
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             53..70
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             671..688
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(741..759)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca
       61 tcatcatcac agcagcggcc tggtgccgcg cggcagccat atggagcagc cgcgtgagga
      121 catcgagcgc ctcgcctccg ccctgctcga agccaagcgc gacctgcacg tctgcccgat
      181 ttgtttcaac atcaccgacg ccgagaagtg cgacgtgtgc gccgacccca gccgcgacca
      241 gcgcaccatc tgcgtggtgg aagaacccgg cgacgtgatt gcgctggagc gcagcggcga
      301 gtaccggggg ctctaccacg tcctgcacgg cgttctgagc ccgatgaacg gcgtcggccc
      361 cgacaagctg cacatcaagc cgctgctgcc ccgcgtgggg caaggcatgg aagtgattct
      421 ggcgactggc acaacggtgg agggcgacgc caccgcgctc taccttcagc ggctgctcga
      481 accgctcggc gccgccatca gccgcatcgc ctacggggtg ccggtgggcg gctcgctgga
      541 atacaccgac gaggtgacgc tgggccgtgc gctcacaggc cgccagaccg tgagcaagcc
      601 ccagccgccg caacgtcccg gtgacgaaga cggcgccgac ggagcagcgg tgcccgcatc
      661 ccgcctcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      721 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      781 ac
//
