LOCUS       sequence-78566-e        5828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniMos transposable element with HygroR, MCS cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1662
FEATURES             Location/Qualifiers
     source          1..5828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    37..57
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    57..164
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     81..97
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     81..97
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(131..147)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(132..148)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(164..184)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(192..209)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(192..208)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3431..3450)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3550..3572
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3610..3628)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3696..3800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3801..3869,3870..4661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4019..4038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4832..5420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5321..5340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgagc
       61 tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
      121 tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccaacttt attatacata
      181 gttgataatt cactggccgt cgttttacag gccggatatg aagatgaacg caaggagcct
      241 gatgcagcga ctcttgctag gagatcatca gaaagcttat atttgattag tgacgtcgca
      301 tcaaagctga acacataaat tctttaatat tttatatccc catattatgc acctgagagg
      361 aatgaagaaa tttgctgata ctcccggtga attcatgtct tgatggttgt gcgcattgtg
      421 gtaatatctg aaaaatttcg aagtcagttt tctgataagg caacaactaa taaattgaga
      481 atcaatttac ccttaaggaa cgatgttcgc ttctgaattt attaataaat ttttccgtaa
      541 ttcaattcaa aattgtcata tttaagaaaa aaacatcaaa aatgacaaat tcaatatttt
      601 aattaatttt tgctttcgtc gtaaatctac acacgcgtct cttccgtgcg agagtccaag
      661 ccagcagcca aattcgttga ctgagtattc aacgtttata cgttgtcggc aacgagaaat
      721 aggaaaatgc atcgggaaat gttctttttt cgattttttc caaggttttg acaaatttta
      781 ccacgaattt tgctatgttt tcaattaaaa aatatgttat tcaactgttt ctatgaggaa
      841 aataaggctt tgcatgtaat tttcttattc agcataattt ttaattaatt tgaattttct
      901 gtcctaacgt ttattttgtt ttcttggtta tgactgatct gaaattaatt tttgaatttt
      961 aaggtaatat gaaaaaaccc gagttgaccg ccacatccgt agagaagttc ctcatcgaga
     1021 agttcgactc cgtctccgac ctcatgcaac tctccgaggg agaggagtcc cgtgccttct
     1081 ccttcgacgt cggaggacgt ggatacgtcc tccgtgtcaa ctcctgcgcc gacggattct
     1141 acaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggacc
     1201 gttacgtcta ccgtcacttc gcctccgccg ccctcccaat cccagaggtc ctcgacatcg
     1261 gagagttctc cgagtccctc acctactgca tctcccgtcg tgcccaagga gtcaccctcc
     1321 aagacctccc agagaccgag ctcccagccg tcctccaacc agtcgccgag gccatggacg
     1381 ccatcgccgc cgccgacctc tcccaaacct ccggattcgg accattcgga ccacaaggaa
     1441 tcggacaata caccacctgg cgtgacttca tctgcgccat cgccgaccca cacgtctacc
     1501 actggcaaac cgtcatggac gacaccgtct ccgcctccgt cgcccaagcc ctcgacgagc
     1561 tcatgctctg ggccgaggac tgcccagagg tccgtcacct cgtccacgcc gacttcggat
     1621 ccaacaacgt cctcaccgac aacggacgta tcaccgccgt catcgactgg tccgaggcca
     1681 tgttcggaga ctcccaatac gaggtcgcca acatcttctt ctggcgtcca tggctcgcct
     1741 gcatggagca acaaacccgt tacttcgagc gtcgtcaccc agagctcgcc ggatccccac
     1801 gtctccgtgc ctacatgctc cgtatcggac tcgaccaact ctaccaatcc ctcgtcgacg
     1861 gaaacttcga cgacgccgcc tgggcccaag gacgttgcga cgccatcgtc cgttccggag
     1921 ccggaaccgt cggacgtacc caaatcgccc gtcgttccgc cgccgtctgg accgacggat
     1981 gcgtcgaggt cctcgccgac tccggaaacc gtcgtccatc cacccgtcca cgtgccaagg
     2041 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gagtaaaatt
     2101 ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc
     2161 gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt
     2221 cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     2281 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     2341 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     2401 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     2461 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     2521 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     2581 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     2641 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     2701 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
     2761 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
     2821 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
     2881 tggagtagtg tctatgggag cttgatccgg atctgcggct tactcaccag acctggcccc
     2941 atccgattac cacctattcg cttcgatggg acacgcactc gctgagcagc gcttcgattc
     3001 ttacgaaagt gtgaaaaaat ggctcgatga atggttcgcc gcaaaagacg atgagttcta
     3061 ctggcgtgga atccacaaat tgcccgagag atgggaaaaa tgtgtagcta gcgacggcaa
     3121 atactttgaa taaatgattt tttctttttc cacaaaattt aacgtgtttt ttgatttaaa
     3181 aaaaacgaca tttcatactt gtacacctga tatctagata cctaggtgag ctctggtacc
     3241 cccctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     3301 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     3361 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     3421 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3481 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3541 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3601 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3661 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4021 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4501 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggtctttt
     5461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5521 ttaccgcctt tgagtgagct gataccgcac cgtacgtctc gaggaattct atcaggtgta
     5581 caagtatgaa atgtcgtttc gaacatatag atgtctcgca aacgtaaata tttatcgatt
     5641 gtcataaaac tttgaccttg tgaagtgtca accttgactg tcgaaccacc atagtttggc
     5701 gcgaattgag cgtcataatt gtttactctc agtgcagtca acatgtcgag tttcgtgccg
     5761 aataaagagc aaacgcggac agtattaatt ttctgttttc atttgaagaa aacagctgcg
     5821 gccggatc
//
