LOCUS       sequence-78568-e        7192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniMos transposable element with HygroR and peel-1, MCS cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 7192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1663
FEATURES             Location/Qualifiers
     source          1..7192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    37..57
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    57..164
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     81..97
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     81..97
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(131..147)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(132..148)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(164..184)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(192..209)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(192..208)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      4328..4651
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(4795..4814)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4914..4936
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4974..4992)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5060..5164
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5165..5233,5234..6025)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5383..5402)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6196..6784
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6685..6704
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgagc
       61 tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattcgata
      121 tcaagcttat cgataccgtc gacctcgagg gggggcccgg taccaacttt attatacata
      181 gttgataatt cactggccgt cgttttacag gccggatatg aagatgaacg caaggagcct
      241 gatgcagcga ctcttgctag gagatcatca gaaagcttat atttgattag tgacgtcgca
      301 tcaaagctga acacataaat tctttaatat tttatatccc catattatgc acctgagagg
      361 aatgaagaaa tttgctgata ctcccggtga attcatgtct tgatggttgt gcgcattgtg
      421 gtaatatctg aaaaatttcg aagtcagttt tctgataagg caacaactaa taaattgaga
      481 atcaatttac ccttaaggaa cgatgttcgc ttctgaattt attaataaat ttttccgtaa
      541 ttcaattcaa aattgtcata tttaagaaaa aaacatcaaa aatgacaaat tcaatatttt
      601 aattaatttt tgctttcgtc gtaaatctac acacgcgtct cttccgtgcg agagtccaag
      661 ccagcagcca aattcgttga ctgagtattc aacgtttata cgttgtcggc aacgagaaat
      721 aggaaaatgc atcgggaaat gttctttttt cgattttttc caaggttttg acaaatttta
      781 ccacgaattt tgctatgttt tcaattaaaa aatatgttat tcaactgttt ctatgaggaa
      841 aataaggctt tgcatgtaat tttcttattc agcataattt ttaattaatt tgaattttct
      901 gtcctaacgt ttattttgtt ttcttggtta tgactgatct gaaattaatt tttgaatttt
      961 aaggtaatat gaaaaaaccc gagttgaccg ccacatccgt agagaagttc ctcatcgaga
     1021 agttcgactc cgtctccgac ctcatgcaac tctccgaggg agaggagtcc cgtgccttct
     1081 ccttcgacgt cggaggacgt ggatacgtcc tccgtgtcaa ctcctgcgcc gacggattct
     1141 acaaggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggacc
     1201 gttacgtcta ccgtcacttc gcctccgccg ccctcccaat cccagaggtc ctcgacatcg
     1261 gagagttctc cgagtccctc acctactgca tctcccgtcg tgcccaagga gtcaccctcc
     1321 aagacctccc agagaccgag ctcccagccg tcctccaacc agtcgccgag gccatggacg
     1381 ccatcgccgc cgccgacctc tcccaaacct ccggattcgg accattcgga ccacaaggaa
     1441 tcggacaata caccacctgg cgtgacttca tctgcgccat cgccgaccca cacgtctacc
     1501 actggcaaac cgtcatggac gacaccgtct ccgcctccgt cgcccaagcc ctcgacgagc
     1561 tcatgctctg ggccgaggac tgcccagagg tccgtcacct cgtccacgcc gacttcggat
     1621 ccaacaacgt cctcaccgac aacggacgta tcaccgccgt catcgactgg tccgaggcca
     1681 tgttcggaga ctcccaatac gaggtcgcca acatcttctt ctggcgtcca tggctcgcct
     1741 gcatggagca acaaacccgt tacttcgagc gtcgtcaccc agagctcgcc ggatccccac
     1801 gtctccgtgc ctacatgctc cgtatcggac tcgaccaact ctaccaatcc ctcgtcgacg
     1861 gaaacttcga cgacgccgcc tgggcccaag gacgttgcga cgccatcgtc cgttccggag
     1921 ccggaaccgt cggacgtacc caaatcgccc gtcgttccgc cgccgtctgg accgacggat
     1981 gcgtcgaggt cctcgccgac tccggaaacc gtcgtccatc cacccgtcca cgtgccaagg
     2041 taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag gagtaaaatt
     2101 ccaactgagc gccggtcgct accattacca acttgtctgg tgtcaaaaat aataggggcc
     2161 gctgtcatca gagtaagttt aaactgagtt ctactaacta acgagtaata tttaaatttt
     2221 cagcatctcg cgcccgtgcc tctgacttct aagtccaatt actcttcaac atccctacat
     2281 gctctttctc cctgtgctcc caccccctat ttttgttatt atcaaaaaac ttctcttaat
     2341 ttctttgttt tttagcttct tttaagtcac ctctaacaat gaaattgtgt agattcaaaa
     2401 atagaattaa ttcgtaataa aaagtcgaaa aaaattgtgc tccctccccc cattaataat
     2461 aattctatcc caaaatctac acaatgttct gtgtacactt cttatgtttt ttacttctga
     2521 taaatttttt tgaaacatca tagaaaaaac cgcacacaaa ataccttatc atatgttacg
     2581 tttcagttta tgaccgcaat ttttatttct tcgcacgtct gggcctctca tgacgtcaaa
     2641 tcatgctcat cgtgaaaaag ttttggagta tttttggaat ttttcaatca agtgaaagtt
     2701 tatgaaatta attttcctgc ttttgctttt tggggtttcc cctattgttt gtcaagattt
     2761 cgaggacggc gtttttcttg ctaaaatcac aagtattgat gagcacgatg caagaaagat
     2821 cggaagaagg tttgggtttg aggctcagtg gaaggtgagt agaagttgat aatttgaaag
     2881 tggagtagtg tctatgggag cttgatccgg atctgcggct tactcaccag acctggcccc
     2941 atccgattac cacctattcg cttcgatggg acacgcactc gctgagcagc gcttcgattc
     3001 ttacgaaagt gtgaaaaaat ggctcgatga atggttcgcc gcaaaagacg atgagttcta
     3061 ctggcgtgga atccacaaat tgcccgagag atgggaaaaa tgtgtagcta gcgacggcaa
     3121 atactttgaa taaatgattt tttctttttc cacaaaattt aacgtgtttt ttgatttaaa
     3181 aaaaacgaca tttcatactt gtacacctga tatctagata cctaggtgag ctctggtacc
     3241 cccctggcgt tacccaactt aatcgccttg cagcagagtc gtattacgta gcccggcctg
     3301 caggtcgact ctagaggatc accaaaaacg gaacgttgag ctggacggaa atagtggtaa
     3361 agtgacatga ttatagtttg aagatttcta atttcacaat tagagcaaat gttgttcggt
     3421 atttattttc aacggtattt atactatttt ccaccttttt ctagaacatt cgagctgctt
     3481 gttgcaaaag gagggcgact cacattcggt acatggaaaa gtagtgtaca caataaagag
     3541 acccagatac attttccgtc tgcgtctctt tgcacccacc gggagtattt tcaaacgaat
     3601 gcatctagga ccttctagaa cattctgtaa ggctgcagaa tgcgggtata taaggaaagc
     3661 gggctcagag gaagccaaca cgctttgttc tagtgcatct aaaaaacttc gaaaatcctc
     3721 atcggatcct atcgattcgc ggccgctgta cacccgggtg ccctatagtg agtcgtattg
     3781 gtaccggtag aaaaaatatg cgctttgatt tccaaaactt aaaattttcg atgattttca
     3841 tatttttgtg gaacatcata gtcggatttt tacttgctct tgtaaagatt ttcaaaatct
     3901 acatgtatct tgatctgcct gaaaatgtat gggtaaatcg gtttgcgcat gttattgctc
     3961 ttattggatc aattgcttca ttgtggttaa ttttttttag cccatttgaa attaagcaag
     4021 gaagattttc tgtctcgtca aaaactatcc tgcttcttgt ttgcaagggt ttgattggct
     4081 tgatatttct tgcacagatt ttcgcccatg tcctttacat tgacccatgc cagaatatgc
     4141 tcttatcatt gaactctgca ctgttctcct tgctgctgaa ttattatgag tctcgtggtg
     4201 cgatccatcc gttgatggaa ttagttcaaa cagctggcct gagaccaact ttgtgcgctg
     4261 ctgtacgatt taattgtctc aactcaactg ctcatattga tccaagtgtt gaaaatccat
     4321 gagataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt
     4381 gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt
     4441 atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgtct
     4501 gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc cgaaaggttg
     4561 agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca acaaaaagag
     4621 gaaactttat tgtgccgcca agaaaaaagt ctcaagctgg cgtaatagcg aagaggcccg
     4681 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     4741 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     4801 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4861 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4921 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     4981 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5041 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5101 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5161 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5221 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5281 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5341 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5401 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5461 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5521 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5581 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5641 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5701 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5761 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5821 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5881 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5941 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6001 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6061 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6121 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6181 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6241 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6301 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     6361 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6421 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6481 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6541 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6601 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6661 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6721 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6781 gaaaaacgcc agcaacgcgg cctttttacg gttcctggtc ttttgctggc cttttgctca
     6841 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6901 agctgatacc gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg
     6961 tttcgaacat atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac
     7021 cttgtgaagt gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat
     7081 aattgtttac tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc
     7141 ggacagtatt aattttctgt tttcatttga agaaaacagc tgcggccgga tc
//
