LOCUS       sequence-78569-e        4853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniMos transposable element with PuroR, MCS cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Sarov M, Taylor J, Flibotte S, LaBella 
            M, Pozniakovsky A, Moerman DG, Jorgensen EM
  TITLE     Random and targeted transgene insertion in Caenorhabditis elegans 
            using a modified Mos1 transposon.
  JOURNAL   Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 
            2014 Mar 16.
  PUBMED    24820376
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2889"; journalName: "Nat Methods"; date: "2014-05"; 
            volume: "11"; issue: "5"; pages: "529-34"
COMMENT     Sequence Label: pCFJ1666
FEATURES             Location/Qualifiers
     source          1..4853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    319..339
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    339..446
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     363..379
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     363..379
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(413..429)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(414..430)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(446..466)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(474..491)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(474..490)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1104..1123)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1702..1722
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2857..2879
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2917..2935)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3003..3107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3108..3176,3177..3968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3326..3345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4139..4727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4628..4647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctatca actttgtata gaaaagttga gctccaccgc ggtggcggcc
      361 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
      421 tcgacctcga gggggggccc ggtaccaact ttattataca tagttgataa ttcactggcc
      481 gtcgttttac agaggggaga aaatgcggag agaagaaccc agcatcttaa taaaattata
      541 taattttgaa taaaatcaaa ttaaaaataa gattaattga aacacaacaa tagaatgcag
      601 aaaattactc ataagaaata atttattttg aaagaagttg tgtcgattta cgataaaaac
      661 tacacgcaaa cgaggctccg cgtttacacc gtttggcaat ggagcgcgct tgctattaga
      721 ttttaggcca gctcaacgaa agtttaattt atttttcttc tttttttcca tttttttgag
      781 taaaatatcg aattttttgt attaattctg taaattttat aatttgcaac tattttggcg
      841 tattctgctt gtttttatca gcgaattttt atgcatttca gtcaaaagtt tgagcaggaa
      901 aaatgacatt taggactttg taaaattgta ttcttaaatt tgcttgaaaa cctacgtgta
      961 caattgattt tattgaattt ttcttacgtt tcccgttgta tcttattgta tttaactttc
     1021 atcataaaat aatttatttc caggtaaaat atccgacgct ctcgtggatc cccgggattg
     1081 gctcagaaaa aaaggtaccg accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     1141 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     1201 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     1261 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cggtaagttt aaacatatat
     1321 atactaacta accctgatta tttaaatttt cagcggacga cggcgccgcg gtggcggtct
     1381 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     1441 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     1501 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gacgtaagtt
     1561 taaacatgat tttactaact aactaatctg atttaaattt tcagcaccag ggcaagggtc
     1621 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     1681 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     1741 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     1801 cctgatgagc tagccggcca tacaagtaat ccggatgatc gacgccaacg tcgttgaatt
     1861 ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat tctctttgtg
     1921 ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt ttttttctct
     1981 ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca agtttattta
     2041 tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg aaacacacaa
     2101 aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat tcagaaatat
     2161 ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaag gtgccggatc
     2221 tgcggcttac tcaccagacc tggccccatc cgattaccac ctattcgctt cgatgggaca
     2281 cgcactcgct gagcagcgct tcgattctta cgaaagtgtg aaaaaatggc tcgatgaatg
     2341 gttcgccgca aaagacgatg agttctactg gcgtggaatc cacaaattgc ccgagagatg
     2401 ggaaaaatgt gtagctagcg acggcaaata ctttgaataa atgatttttt ctttttccac
     2461 aaaatttaac gtgttttttg atttaaaaaa aacgacattt catacttgta cacctgatat
     2521 ctagatacct aggtgagctc tggtaccccc ctggcgttac ccaacttaat cgccttgcag
     2581 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2641 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     2701 tgtgcggtat ttcacaccgc atatggtgca ctctcagtgc aatctgctct gatgccgcat
     2761 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2821 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2881 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     2941 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3001 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3061 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3121 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3181 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3241 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3301 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3361 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3421 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3481 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3541 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3601 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3661 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3721 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3781 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3841 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3901 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3961 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4021 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4081 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4141 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4201 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4261 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4321 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4381 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4441 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4501 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4561 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4621 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4681 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4741 cggccttttt acggttcctg gtcttttgct ggccttttgc tcacatgttc tttcctgcgt
     4801 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat acc
//
