LOCUS       sequence-78583-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for
            DSB mediated EGFP reconstitution. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, Ikawa M
  TITLE     Generation of mutant mice by pronuclear injection of circular 
            plasmid expressing Cas9 and single guided RNA.
  JOURNAL   Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355.
  PUBMED    24284873
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-27"; volume: "3:3355. doi"; pages: " 
            10.1038/srep03355"
COMMENT     Sequence Label: pCAG-EGxxFP-Cetn1
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      48..57
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(54..56,57..59,60..572)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(99..120)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(360..379)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa atctagagcc gccatggtga
       61 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      121 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      181 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      241 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      301 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      361 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      421 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      481 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      541 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      601 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactgaggat
      661 ccagtcactt aataaaggtt ggtctttttc atgatcttaa gaaactcttc ctcgttcact
      721 tctccgtcgc catcacgatc agcttcatca atcatttcct gcagctcctc gtctgtgagg
      781 ctttccccca gctcattggc cacacgcttg aggtttttga atgaaatttt cccagtttcg
      841 tcgtcatcaa acaacctgaa agccttcagg atttcctctt tggtatcttt ctcggccatc
      901 ttctgagtca tcacagccaa gaagtcattg aagctgatct ttcctgtggc ctctttgtct
      961 acttctgaaa tcattttctt catctcttcc ttcctgggtt caaagccaag tgctctcatg
     1021 gccaccttca gctccttc
//
