LOCUS       sequence-78588-e       12958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA destination plasmid. Contains the bean yellow dwarf virus 
            cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. 
            Contains attR1 and attR2 sites between the LIR and SIR sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12958)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 12958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSL
FEATURES             Location/Qualifiers
     source          1..12958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    529..653
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(678..683,684..701,702..708)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             762..1442
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(828..847)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1762..2067
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1946..1965
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2108..2232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2999..3343
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(4055..4071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4055..4071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4068..4090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4079..4095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4103..4109,4110..4127,4128..4133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4148..4169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4360..5037
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             5105..6130
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    6171..6345
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6423..6447
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6872..7666
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7753..8341
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8242..8261
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8495..8512
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8527..8667
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8593..8612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8753..8775
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9011..9205
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9271..10338)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10772..11401)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12701..12725
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     12913..12935
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12927..12944
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12928..12944
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggta gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt
       61 ataaccggcg tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa
      121 atgacgtgga atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt
      181 taatattacc ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt
      241 aaagtagaaa atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg
      301 atgtgatggt atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat
      361 tcgtacgacc ctccctaaga ttcttgattg tttataaaac caaatctcat tgtctttgtt
      421 gtgtattgtt tgcaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
      481 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttctac aagtttgtac
      541 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      601 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
      661 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
      721 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      781 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      841 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      901 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      961 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     1021 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     1081 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     1141 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     1201 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     1261 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     1321 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     1381 aattacaaca gtactgcgat gagtggcagg cggggcgtaa tctagaggat ccggcttact
     1441 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     1501 tgatatgtat acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg
     1561 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     1621 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     1681 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     1741 acgagaacag gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     1801 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     1861 atccccctgg ccagtgcacg tctgctgtca gataaagtcc cccgtgaact ttacccggtg
     1921 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     1981 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     2041 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     2101 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     2161 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     2221 gtacaaagtg gtgagtgtac ttcaagtcag tgggaaatca ataaaatgat tattttatga
     2281 atatatttca ttgtgcaagt agatagaaat tacatatgtt acataacaca cgaaataaac
     2341 aaaaaaagac aatccaaaaa caaacacccc aaaaaaaata atcactttag ataaactcgt
     2401 atgaggagag gcacgttcag tgactcgacg attcccgagc aaaaaaagtc tccccgtcac
     2461 acatgtagtg ggtgacgcaa ttatctttaa agtaatcctt ctgttgactt gtcattgata
     2521 acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg gatcccactc gagccaagct
     2581 tggcgtgtct gcaggtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
     2641 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     2701 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     2761 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     2821 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     2881 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgataacatg gtggagcacg
     2941 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     3001 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     3061 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     3121 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     3181 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     3241 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     3301 acccttcctc tatataagga agttcatttc atttggagag gacctcgact ctagaggatc
     3361 ccccttcctc tatataagga agttcatttc atttggagag gtaagtttca cttcacacat
     3421 tattactgtc ttctaataca aggtttttta tcaagctgga gaagagcatg atagtgggta
     3481 gtgccatctt gatgaagctc agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt
     3541 ttctcccaga gaaactggaa taaatcatct ctttgagatg agcacttggg ataggtaagg
     3601 aaaacatatt tagattggag tctgaagttc ttactagcag aaggcatgtt gttgtgactc
     3661 cgaggggttg cctcaaactc tatcttataa ccggcgtgga ggcatggagg caggggtatt
     3721 ttggtcattt taatagatag tggaaaatga cgtggaattt acttaaagac gaagtctttg
     3781 cgacaagggg gggcccacgc cgaatttaat attaccggcg tggccccccc ttatcgcgag
     3841 tgctttagca cgagcggtcc agatttaaag tagaaaattt cccgcccact agggttaaag
     3901 gtgttcacac tataaaagca tatacgatgt gatggtattt gatggagcgt atattgtatc
     3961 aggtatttcc gttggatacg aattattcgt acgaccctcc ctgcaggtcg actctagagg
     4021 atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct gtgtgaaatt
     4081 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     4141 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     4201 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4261 tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg tctactccaa
     4321 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     4381 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     4441 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     4501 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     4561 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt
     4621 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     4681 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     4741 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     4801 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     4861 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     4921 gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc
     4981 ttcgcaagac cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat
     5041 caccagtctc tctctacaaa tctatctctc tcgagctttc gcagatccgg gggggcaatg
     5101 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     5161 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     5221 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     5281 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     5341 cattggggag tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     5401 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttcta caaccggtcg cggaggctat
     5461 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     5521 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     5581 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     5641 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     5701 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     5761 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     5821 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     5881 gccacgactc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     5941 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     6001 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     6061 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     6121 aaagaaatag agtagatgcc gacgcggatc tgtcgatcga caagctcgag tttctccata
     6181 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
     6241 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     6301 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg
     6361 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
     6421 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
     6481 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
     6541 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
     6601 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
     6661 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
     6721 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
     6781 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
     6841 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
     6901 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
     6961 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
     7021 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
     7081 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
     7141 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
     7201 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
     7261 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
     7321 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
     7381 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     7441 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     7501 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     7561 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     7621 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat
     7681 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7741 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7801 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7861 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7921 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7981 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8041 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8101 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8161 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8221 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8281 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8341 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8401 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8461 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8521 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     8581 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     8641 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     8701 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     8761 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     8821 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     8881 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     8941 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     9001 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     9061 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     9121 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     9181 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     9241 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     9301 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     9361 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     9421 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     9481 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     9541 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     9601 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
     9661 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
     9721 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
     9781 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
     9841 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
     9901 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
     9961 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    10021 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    10081 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    10141 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    10201 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    10261 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    10321 gccggatggt ttgcgaccgt cacgccgatt cctcgggctt gggggttcca gtgccattgc
    10381 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
    10441 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
    10501 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
    10561 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
    10621 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
    10681 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
    10741 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
    10801 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
    10861 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
    10921 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
    10981 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
    11041 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
    11101 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
    11161 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
    11221 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
    11281 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
    11341 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
    11401 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
    11461 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
    11521 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
    11581 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
    11641 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
    11701 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
    11761 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
    11821 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
    11881 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
    11941 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
    12001 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
    12061 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    12121 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    12181 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    12241 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    12301 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    12361 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    12421 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    12481 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    12541 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
    12601 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
    12661 ttaaatatcc gttattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
    12721 tgtcaaacac tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc
    12781 tgctctagca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    12841 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    12901 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatg
//
