LOCUS       sequence-78589-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full length PFD1130w.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Zenonos ZA, Rayner JC, Wright GJ
  TITLE     Towards a comprehensive Plasmodium falciparum merozoite cell surface
            and secreted recombinant protein library.
  JOURNAL   Malar J. 2014 Mar 12;13(1):93. doi: 10.1186/1475-2875-13-93.
  PUBMED    24620899
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2014-03-12"; volume: "13(1):93. doi"; pages: " 
            10.1186/1475-2875-13-93"
COMMENT     Sequence Label: PFD1130w-bio-His
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     204..223
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    222..277
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(329..346)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gaaccccttt gtgtttggtc cccccccgtg tcacatgtgg aacagggccc agttggcaag
       61 ttgtaccaac caactgaagg gattacatgc actgccccgc gaagaagggg cagagatgtc
      121 gtagtcaggt ttagttcgtc cggggcgggg atcgatcctc tagctagaga tctccgaggg
      181 atctcgacca aatgatttgc cctcccatat gtccttccga gtgagagaca caaaaaattc
      241 caacacacta ttgcaatgaa aataaatttc ctttattagc cagaggtcga ggtcggggga
      301 tccttaatta atcacgtgtc tagactcaat ggtggtgatg gtggtggggg cccagagggg
      361 ccagaggagg caggttcctg tccctgtggt tccacagcat cttctgggcg tccaggatgt
      421 ggtgcaggct gccggaattc aaccctttgg ataaaacctg gatcttggag tccatcttga
      481 cctcttcacc ttcactcagt agacattgcc acacccctgc ttcaggggcc tgcacttgaa
      541 tcactttctc ctgcctggag accctggcct cctgattctc ctgcttcaag atcagtctca
      601 tcttgggtga ggtgggtccc atcacctcac aggtcaaagt gttgctgtcg ggctgagtca
      661 ctttcatcac caccaggttc acttcctgat acagtatccc tctgtccaga gtcagggtca
      721 ggttgccaga accagcaaac tgaagggaga cctggggtat ctgaagggtg agtgggagcg
      781 tttcggacag ctggaacttg gggttgctag tagacttctg cacagacacc ttttggttct
      841 ttagggagaa ggtgatccag gactgggaag aa
//
