LOCUS       sequence-78590-e       15194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLSL with GFP followed by the us:NPTII repair template, Rep is not 
            included on this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15194)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 15194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLGFP
FEATURES             Location/Qualifiers
     source          1..15194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    529..553
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             575..640
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             647..667
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             758..1468
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(785..813)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1408..1428
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1491..1743
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    1788..1808
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3285..4076
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3336..3355)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3946..3965
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4103..4355
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4379..4398)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4380..4398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4445..4469)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5235..5579
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6291..6307)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6291..6307)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6304..6326)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6315..6331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6339..6345,6346..6363,6364..6369))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6384..6405
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6596..7273
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             7341..8366
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    8407..8581
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8659..8683
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9108..9902
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9989..10577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10478..10497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10731..10748
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10763..10903
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10829..10848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10989..11011
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11247..11441
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11507..12574)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(13008..13637)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    14937..14961
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     15149..15171
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15163..15180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15164..15180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggta gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt
       61 ataaccggcg tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa
      121 atgacgtgga atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt
      181 taatattacc ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt
      241 aaagtagaaa atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg
      301 atgtgatggt atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat
      361 tcgtacgacc ctccctaaga ttcttgattg tttataaaac caaatctcat tgtctttgtt
      421 gtgtattgtt tgcaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
      481 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttctac aagtttgtac
      541 aaaaaagcag gctccgaatt cgcccttcac catggattat aaggatcacg atggcgacta
      601 caaggaccac gatattgact acaaagacga tgacgataaa atggctccta agaaaaagcg
      661 caaagtcggt atccatggcg ttccctctag aatgaagact aatctttttc tctttctcat
      721 cttttcactt ctcctatcat tatcctcggc cgaattcagt aaaggagaag aacttttcac
      781 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
      841 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac
      901 tactggaaaa ctacctgttc catggccaac acttgtcact actttctctt atggtgttca
      961 atgcttttca agatacccag atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc
     1021 tgagggatac gtgcaggaga ggaccatctt cttcaaggac gacgggaact acaagacacg
     1081 tgctgaagtc aagtttgagg gagacaccct cgtcaacagg atcgagctta agggaatcga
     1141 tttcaaggag gacggaaaca tcctcggcca caagttggaa tacaactaca actcccacaa
     1201 cgtatacatc atggccgaca agcaaaagaa cggcatcaaa gccaacttca agacccgcca
     1261 caacatcgaa gacggcggcg tgcaactcgc tgatcattat caacaaaata ctccaattgg
     1321 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa
     1381 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat
     1441 tacacatggc atggatgaac tatacaaaca tgatgagctt taagacgtcc gatcgttcaa
     1501 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     1561 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     1621 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     1681 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     1741 atcgggaatt gatcccccct cgacagcttc cggaaagggc gaattcgcaa ctttgtatac
     1801 aaaagttgcc gagctcgcgg ccgcagcagt cttacttcca tgatttcttt aactatgccg
     1861 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
     1921 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
     1981 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
     2041 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
     2101 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg
     2161 tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct
     2221 actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg
     2281 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     2341 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     2401 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca
     2461 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     2521 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     2581 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     2641 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     2701 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     2761 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     2821 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     2881 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     2941 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     3001 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     3061 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     3121 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaacgcagg gaggcaaaca
     3181 atgatatcac aactctcctg acgcgtcatc gtcggctaca gcctcgggaa ttgctaccta
     3241 gctcgagcaa gatccaagga gatataacaa tggcttcctc ctggattgaa caagatggat
     3301 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     3361 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggt agaccggttc
     3421 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     3481 tatcgtggct ggccacgacg ggcgtacctt gcgctgctgt gctcgacgtt gtcactgaag
     3541 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     3601 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     3661 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     3721 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     3781 cagccgaact gttcgccagg ctcaaggcga gaatgcccga cggcgaggat ctcgtcgtga
     3841 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     3901 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     3961 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     4021 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgataac
     4081 cgcggagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     4141 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     4201 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     4261 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     4321 attatcgcgc gcggtgtcat ctatgttact agatcgtggg cccgtccagt tccattcgcc
     4381 ctatagtgag tcgtattaaa cctgcattaa tgaatcggcc aacgcgcggg gctgcaggtc
     4441 gacgacccag ctttcttgta caaagtggga gtgtacttca agtcagtggg aaatcaataa
     4501 aatgattatt ttatgaatat atttcattgt gcaagtagat agaaattaca tatgttacat
     4561 aacacacgaa ataaacaaaa aaagacaatc caaaaacaaa caccccaaaa aaaataatca
     4621 ctttagataa actcgtatga ggagaggcac gttcagtgac tcgacgattc ccgagcaaaa
     4681 aaagtctccc cgtcacacat gtagtgggtg acgcaattat ctttaaagta atccttctgt
     4741 tgacttgtca ttgataacat ccagtcttcg tcaggattgc aaagaattat agaagggatc
     4801 ccactcgagc caagcttggc gtgtctgcag gtcaacatgg tggagcacga cacacttgtc
     4861 tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa
     4921 caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt
     4981 gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
     5041 gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
     5101 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     5161 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
     5221 gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc
     5281 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac
     5341 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt
     5401 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     5461 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
     5521 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggacc
     5581 tcgactctag aggatccccc ttcctctata taaggaagtt catttcattt ggagaggtaa
     5641 gtttcacttc acacattatt actgtcttct aatacaaggt tttttatcaa gctggagaag
     5701 agcatgatag tgggtagtgc catcttgatg aagctcagaa gcaacaccaa ggaagaaaat
     5761 aagaaaaggt gtgagtttct cccagagaaa ctggaataaa tcatctcttt gagatgagca
     5821 cttgggatag gtaaggaaaa catatttaga ttggagtctg aagttcttac tagcagaagg
     5881 catgttgttg tgactccgag gggttgcctc aaactctatc ttataaccgg cgtggaggca
     5941 tggaggcagg ggtattttgg tcattttaat agatagtgga aaatgacgtg gaatttactt
     6001 aaagacgaag tctttgcgac aagggggggc ccacgccgaa tttaatatta ccggcgtggc
     6061 ccccccttat cgcgagtgct ttagcacgag cggtccagat ttaaagtaga aaatttcccg
     6121 cccactaggg ttaaaggtgt tcacactata aaagcatata cgatgtgatg gtatttgatg
     6181 gagcgtatat tgtatcaggt atttccgttg gatacgaatt attcgtacga ccctccctgc
     6241 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg gtcatagctg
     6301 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6361 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6421 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6481 gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca
     6541 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     6601 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     6661 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     6721 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     6781 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     6841 gatgtgataa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
     6901 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
     6961 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
     7021 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
     7081 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     7141 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     7201 cacaatccca ctatccttcg caagaccttc ctctatataa ggaagttcat ttcatttgga
     7261 gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga gctttcgcag
     7321 atccgggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     7381 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     7441 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     7501 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     7561 ttccggaagt gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc
     7621 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac
     7681 cggtcgcgga ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     7741 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     7801 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     7861 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     7921 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     7981 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     8041 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     8101 cggagcttgc aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac
     8161 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     8221 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     8281 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     8341 gcactcgtcc gagggcaaag aaatagagta gatgccgacg cggatctgtc gatcgacaag
     8401 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
     8461 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     8521 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
     8581 cccccgaatt aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
     8641 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
     8701 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
     8761 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
     8821 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
     8881 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc
     8941 tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg
     9001 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct
     9061 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga
     9121 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
     9181 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
     9241 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
     9301 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
     9361 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
     9421 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
     9481 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
     9541 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat
     9601 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
     9661 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
     9721 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
     9781 tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
     9841 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
     9901 agtacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     9961 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10021 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10081 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    10141 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10201 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10261 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10321 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10381 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10441 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10501 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10561 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10621 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10681 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10741 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
    10801 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    10861 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
    10921 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    10981 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
    11041 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
    11101 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
    11161 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
    11221 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
    11281 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
    11341 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
    11401 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga ccttttcgac
    11461 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
    11521 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
    11581 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
    11641 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
    11701 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
    11761 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
    11821 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
    11881 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
    11941 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
    12001 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
    12061 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
    12121 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
    12181 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
    12241 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
    12301 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
    12361 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
    12421 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
    12481 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
    12541 cgccgatttg taccgggccg gatggtttgc gaccgtcacg ccgattcctc gggcttgggg
    12601 gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc
    12661 gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg
    12721 ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg
    12781 tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta
    12841 catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg
    12901 cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc
    12961 acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt
    13021 gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat
    13081 tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg
    13141 gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc
    13201 gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca
    13261 atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc
    13321 tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc
    13381 tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca
    13441 atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa
    13501 tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc
    13561 agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt
    13621 acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat
    13681 acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc
    13741 ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca
    13801 aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt
    13861 acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc
    13921 cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc
    13981 gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt
    14041 cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc
    14101 agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg
    14161 tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg
    14221 cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac
    14281 ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg
    14341 caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg
    14401 ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat
    14461 cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt
    14521 gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac
    14581 gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt
    14641 ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa
    14701 gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga
    14761 cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt
    14821 actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
    14881 accttttcac gcccttttaa atatccgtta ttctaataaa cgctcttttc tcttaggttt
    14941 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
    15001 tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg
    15061 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    15121 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    15181 gccaagcttg catg
//
