LOCUS       sequence-78591-e         990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression vector for yeast, N152,C18 architecture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY566
FEATURES             Location/Qualifiers
     source          1..990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             129..149
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             150..176
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
     CDS             complement(483..890)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     740..762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     754..771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     755..771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     781..800
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        781..799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(847..867)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(847..865)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(886..902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(886..902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(899..921)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    910..926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(934..940,941..958,959..964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt
       61 agatctaact cgagaaagat attgtatata tcgtaacaat aggaggttca acaatggctt
      121 cctcccctcc aaagaaaaag agaaaggtta gttggaagga cgcaagtggt tggtctagaa
      181 tggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc aaaccgaagg
      241 tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt acacacgcgc
      301 acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc acgtatcagc
      361 acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc ggcaaacagt
      421 ggtccggcgc acgcgccctg gaggccttgc tcacggatgc gggggagttg agaggtccgc
      481 cgttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc gtgaccgcaa
      541 tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc ccccctggag acgggcgccg
      601 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
      661 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      721 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
      781 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt
      841 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
      901 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      961 taaagcctgg ggtgcctaat gagtgagcta
//
