LOCUS       sequence-78592-e       15655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLSL with Zif268:FokI coding sequence followed by approximately 3kb 
            'filler' sequence, Rep is not included on this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15655)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 15655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLZ
FEATURES             Location/Qualifiers
     source          1..15655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    529..553
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             587..607
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             878..1462
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
                     VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      1476..1728
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    1773..1793
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2824..3615
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2875..2894)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3485..3504
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      3642..3894
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    complement(4907..4931)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5697..6041
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6752..6768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6752..6768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6765..6787)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6776..6792
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6800..6806,6807..6824,6825..6830))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6845..6866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7057..7734
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             7802..8827
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    8868..9042
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9120..9144
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9569..10363
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10450..11038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10939..10958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11192..11209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11224..11364
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11290..11309)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11450..11472
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11708..11902
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11968..13035)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(13469..14098)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    15398..15422
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     15610..15632
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15624..15641
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15625..15641
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggta gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt
       61 ataaccggcg tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa
      121 atgacgtgga atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt
      181 taatattacc ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt
      241 aaagtagaaa atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg
      301 atgtgatggt atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat
      361 tcgtacgacc ctccctaaga ttcttgattg tttataaaac caaatctcat tgtctttgtt
      421 gtgtattgtt tgcaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
      481 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttctac aagtttgtac
      541 aaaaaagcag gctccgaatt cgcccttcac catggcttcc tcccctccaa agaaaaagag
      601 aaaggttctt gaattgcctt acgcttgtcc agttgagtct tgtgatagac gttttagtag
      661 gtcagacgaa ctcactaggc atattagaat ccacacagga cagaagcctt tccagtgtag
      721 aatctgtatg aggaacttta gtcgtagcga tcatttgact acacatatca gaacccatac
      781 tggtgagaaa ccattcgcat gtgacatctg tggtcgtaag ttcgcaagaa gtgatgaaag
      841 gaagcgtcat acaaagatcc atcttcgtca gaaggaacta gtgaaatctg aattggaaga
      901 gaagaaatct gaacttagac ataaattgaa atatgtgcca catgaatata ttgaattgat
      961 tgaaatcgca agaaattcaa ctcaggatag aatccttgaa atgaaggtga tggagttctt
     1021 tatgaaggtt tatggttatc gtggtaaaca tttgggtgga tcaaggaaac cagacggagc
     1081 aatttatact gtcggatctc ctattgatta cggtgtgatc gttgatacta aggcatattc
     1141 aggaggttat aatcttccaa ttggtcaagc agatgaaatg caaagatatg tcgaagagaa
     1201 tcaaacaaga aacaagcata tcaaccctaa tgaatggtgg aaagtctatc catcttcagt
     1261 aacagaattt aagttcttgt ttgtgagtgg tcatttcaaa ggaaactaca aagctcagct
     1321 tacaagattg aatcatatca ctaattgtaa tggagctgtt cttagtgtag aagagctttt
     1381 gattggtgga gaaatgatta aagctggtac attgacactt gaggaagtga gaaggaaatt
     1441 taataacggt gagataaact tttaatagga cgtccgatcg ttcaaacatt tggcaataaa
     1501 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     1561 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     1621 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     1681 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc
     1741 cccctcgaca gcttccggaa agggcgaatt cgcaactttg tatacaaaag ttgccgagct
     1801 cgcggccgca gtgcgagatc gggccggcct ggccctgttt tgattgatgt acctaaggat
     1861 attcagcaac aattggtgat acctgactgg gatcagccaa tgaggttgcc tggttacatg
     1921 tctaggttac ctaaattgcc caatgagatg cttttagaac aaattgttag gcttatttct
     1981 gagtcaaaga agcctgtttt gtatgtgggg ggtgggtgtt cgcaatcgag tgaggagttg
     2041 agacgattcg tggagctcac cggtatcccc gtggcaagta ctttgatggg tcttggagct
     2101 tttccaactg gggatgagct ttccctttca atgttgggta tgcatggtac tgtttatgct
     2161 aattatgctg tggacagtag tgatttattg ctcgcatttg gggtgaggtt tgatgataga
     2221 gttactggaa agttagaagc ttttgctagc cgagcgaaaa ttgttcacat tgatattgat
     2281 tcagctgaga ttggaaagaa caagcagcct catgtttcca tttgtgcgga tatcaagttg
     2341 gcgttacagg gtttgaattc gatattggag agtaaggaag gtaaactgaa gttggatttt
     2401 tctgcttgga ggcaggagtt gacggtgcag aaagtgaagt acccgttgaa ttttaaaact
     2461 tttggtgatg ctattcctcc gcaatatgct atccaggttc tagatgagtt aactaatggg
     2521 agtgctatta taagtaccgg tgttgggcag caccagatgt gggctgctca atattataag
     2581 tacagaaagc cacgccaatg gttgacatct ggtggattag gagcgatggg atttggtttg
     2641 cccgctgcta ttggtgcggc tgttggaaga cctgatgaag ttgtggttga cattgatggt
     2701 gatggcagtt tcatcatgaa tgtgcaggag ctagcaacta ttaaggtgga gaatctccca
     2761 gttaagatta tgttactgaa taatcaacac ttgggaatgg ttgtacaaat ggcttcctcc
     2821 tggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     2881 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     2941 gcgcagggta gaccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3001 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgtaccttg cgctgctgtg
     3061 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3121 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3181 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3241 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3301 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag aatgcccgac
     3361 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3421 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3481 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3541 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     3601 gacgagttct tctgataacc gcggagagct cgaatttccc cgatcgttca aacatttggc
     3661 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     3721 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     3781 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     3841 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgtgggc
     3901 ctcgaggatc gattctataa ggctaacaga gcacacacat acctggggaa tccttctaat
     3961 gaggcggaga tctttcctaa tatgttgaaa tttgcagagg cttgtggcgt acctgctgcg
     4021 agagtgacac acagggatga tcttagagcg gctattcaaa agatgttaga cactcctggg
     4081 ccatacttgt tggatgtgat tgtacctcat caggaacatg ttctacctat gattcctacc
     4141 ggaggggctt tcaaagacgt catcacagag ggtgacggga gaagttccta ttgactttga
     4201 ggtgctacag agctagttct aggccttgta ttatctaaaa taaacttcta ttaaaccaaa
     4261 aatgttatgt ctattagttt gttattagtt tttccgtggc tttgctcatt gtcagtgttg
     4321 tactattaag tagttgatat ttatgtttgc tttaagtttt gcatcatctc gctttggttt
     4381 tgaatgtgaa ggatttcagc aatgtttcat tctctattcg caacatccag tcggtatccg
     4441 gagctctatg tagtatgtct ggagattaat ttctagtgga gtagtttagt gcgataaagt
     4501 tagcttgttc cacattttta tttcgtaacc tgggtcagat tggaacttcc tctttaggtt
     4561 ggatgcaatc cctatttggg ctttctctta atttcattat tgaaattgtt ggcttttaat
     4621 ctgagcaagt tgatttgcag ctttctctct tgagtcctag cgagcaatac gttatctctg
     4681 tctcctattt cttagtggat aatcttatga tggaaatctg tggagatagg aaactgttga
     4741 ctgctcattc tctctttgtg aggcgtctga cagttacgct ttcaatctat agcagttcga
     4801 tcagactttc tttgcttata acaatactag atacagttgg accgtgtagc acgggcccaa
     4861 caatttgtgt aacgttatct ttaattcaaa atatcgcagg tcgacgaccc agctttcttg
     4921 tacaaagtgg gagtgtactt caagtcagtg ggaaatcaat aaaatgatta ttttatgaat
     4981 atatttcatt gtgcaagtag atagaaatta catatgttac ataacacacg aaataaacaa
     5041 aaaaagacaa tccaaaaaca aacaccccaa aaaaaataat cactttagat aaactcgtat
     5101 gaggagaggc acgttcagtg actcgacgat tcccgagcaa aaaaagtctc cccgtcacac
     5161 atgtagtggg tgacgcaatt atctttaaag taatccttct gttgacttgt cattgataac
     5221 atccagtctt cgtcaggatt gcaaagaatt atagaaggga tcccactcga gccaagcttg
     5281 gcgtgtctgc aggtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
     5341 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
     5401 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
     5461 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
     5521 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     5581 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg ataacatggt ggagcacgac
     5641 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
     5701 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
     5761 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
     5821 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
     5881 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     5941 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     6001 ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgactct agaggatccc
     6061 ccttcctcta tataaggaag ttcatttcat ttggagaggt aagtttcact tcacacatta
     6121 ttactgtctt ctaatacaag gttttttatc aagctggaga agagcatgat agtgggtagt
     6181 gccatcttga tgaagctcag aagcaacacc aaggaagaaa ataagaaaag gtgtgagttt
     6241 ctcccagaga aactggaata aatcatctct ttgagatgag cacttgggat aggtaaggaa
     6301 aacatattta gattggagtc tgaagttctt actagcagaa ggcatgttgt tgtgactccg
     6361 aggggttgcc tcaaactcta tcttataacc ggcgtggagg catggaggca ggggtatttt
     6421 ggtcatttta atagatagtg gaaaatgacg tggaatttac ttaaagacga agtctttgcg
     6481 acaagggggg gcccacgccg aatttaatat taccggcgtg gccccccctt atcgcgagtg
     6541 ctttagcacg agcggtccag atttaaagta gaaaatttcc cgcccactag ggttaaaggt
     6601 gttcacacta taaaagcata tacgatgtga tggtatttga tggagcgtat attgtatcag
     6661 gtatttccgt tggatacgaa ttattcgtac gaccctccct gcaggtcgac tctagaggat
     6721 ccccgggtac cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     6781 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6841 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6901 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6961 gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct actccaagaa
     7021 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     7081 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     7141 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     7201 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     7261 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata acatggtgga
     7321 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
     7381 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
     7441 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
     7501 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
     7561 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
     7621 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
     7681 gcaagacctt cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac
     7741 cagtctctct ctacaaatct atctctctcg agctttcgca gatccggggg ggcaatgaga
     7801 tatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     7861 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     7921 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     7981 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     8041 tggggagttt agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     8101 gcaagacctg cctgaaaccg aactgcccgc tgttctacaa ccggtcgcgg aggctatgga
     8161 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
     8221 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
     8281 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
     8341 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
     8401 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
     8461 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
     8521 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
     8581 acgactccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
     8641 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
     8701 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
     8761 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
     8821 gaaatagagt agatgccgac gcggatctgt cgatcgacaa gctcgagttt ctccataata
     8881 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
     8941 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     9001 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcccccgaat taattcggcg
     9061 ttaattcagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     9121 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     9181 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     9241 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     9301 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     9361 agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat
     9421 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
     9481 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
     9541 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
     9601 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
     9661 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
     9721 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
     9781 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
     9841 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
     9901 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
     9961 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
    10021 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
    10081 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
    10141 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
    10201 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
    10261 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
    10321 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac
    10381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    10441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    10501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    10561 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
    10621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    10681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    10741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    10801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    10861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    10921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    10981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    11041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    11101 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    11161 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    11221 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
    11281 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg
    11341 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
    11401 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
    11461 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg
    11521 gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg
    11581 taggccagcc atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg
    11641 tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc
    11701 agacagttat gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta
    11761 ggcggaaaaa tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc
    11821 aatgtacggc tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt
    11881 cccaatgtac gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc
    11941 aatttgccct agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag
    12001 gttgcggtag cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta
    12061 ctccggcagg tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc
    12121 tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc
    12181 tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta
    12241 atcggggtga accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca
    12301 atcagctagc tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta
    12361 gcggctaatc aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt
    12421 cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc
    12481 tttctgcttt ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg
    12541 gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag
    12601 atgggaaacc gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc
    12661 tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg
    12721 gtcacgcttc gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc
    12781 cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct
    12841 aatcgacggc gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc
    12901 cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg
    12961 cgcggccttc aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc
    13021 ggatggtttg cgaccgtcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    13081 gccggcagac aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    13141 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    13201 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    13261 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    13321 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    13381 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    13441 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    13501 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    13561 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    13621 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    13681 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    13741 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    13801 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    13861 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    13921 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    13981 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    14041 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    14101 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    14161 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    14221 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    14281 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    14341 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    14401 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    14461 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    14521 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    14581 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    14641 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    14701 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    14761 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    14821 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    14881 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    14941 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    15001 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    15061 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    15121 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    15181 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    15241 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    15301 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
    15361 aatatccgtt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
    15421 caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
    15481 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
    15541 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
    15601 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatg
//
