LOCUS       sequence-78593-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ePathBrick vector for pathway engineering in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Xu P, Vansiri A, Bhan N, Koffas MA
  TITLE     ePathBrick: a synthetic biology platform for engineering metabolic 
            pathways in E. coli.
  JOURNAL   ACS Synth Biol. 2012 Jul 20;1(7):256-66. doi: 10.1021/sb300016b. 
            Epub 2012 May 4.
  PUBMED    23651248
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb300016b";
            journalName: "ACS Synth Biol"; date: "2012-07-20- 20"; volume: "1"; 
            issue: "7"; pages: "256-66"
COMMENT     Sequence Label: pRSM3
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..43
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             154..198
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(237..255)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      251..298
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg gcagatctca attggatatc ggccggccac
      121 gcgatcgctg acgtcggtac cctcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa
      181 cgccagcaca tggactcgtc tactagtcgc agcttaatta acctaaactg ctgccaccgc
      241 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      301 agcgaaagga ggagtcgaca agctgacgac cgggtctccg caagtggcac ttttcgggga
      361 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      421 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
      481 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
      541 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
      601 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
      661 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
      721 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
      781 cattcgtgat tgcgcctga
//
