LOCUS       sequence-78596-e       15179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLSL with Zif268:FokI coding sequence followed by us:NPTII repair 
            template , Rep is not included on this plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15179)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 15179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLZ.D
FEATURES             Location/Qualifiers
     source          1..15179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    529..553
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             587..607
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             878..1462
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
                     VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      1476..1728
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    1773..1793
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3270..4061
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3321..3340)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3931..3950
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4088..4340
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4364..4383)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4365..4383)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4430..4454)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5220..5564
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6276..6292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6276..6292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6289..6311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6300..6316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6324..6330,6331..6348,6349..6354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6369..6390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6581..7258
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             7326..8351
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    8392..8566
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8644..8668
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9093..9887
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9974..10562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10463..10482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10716..10733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10748..10888
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10814..10833)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10974..10996
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11232..11426
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11492..12559)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(12993..13622)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    14922..14946
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     15134..15156
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15148..15165
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15149..15165
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cctgcaggta gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt
       61 ataaccggcg tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa
      121 atgacgtgga atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt
      181 taatattacc ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt
      241 aaagtagaaa atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg
      301 atgtgatggt atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat
      361 tcgtacgacc ctccctaaga ttcttgattg tttataaaac caaatctcat tgtctttgtt
      421 gtgtattgtt tgcaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
      481 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttctac aagtttgtac
      541 aaaaaagcag gctccgaatt cgcccttcac catggcttcc tcccctccaa agaaaaagag
      601 aaaggttctt gaattgcctt acgcttgtcc agttgagtct tgtgatagac gttttagtag
      661 gtcagacgaa ctcactaggc atattagaat ccacacagga cagaagcctt tccagtgtag
      721 aatctgtatg aggaacttta gtcgtagcga tcatttgact acacatatca gaacccatac
      781 tggtgagaaa ccattcgcat gtgacatctg tggtcgtaag ttcgcaagaa gtgatgaaag
      841 gaagcgtcat acaaagatcc atcttcgtca gaaggaacta gtgaaatctg aattggaaga
      901 gaagaaatct gaacttagac ataaattgaa atatgtgcca catgaatata ttgaattgat
      961 tgaaatcgca agaaattcaa ctcaggatag aatccttgaa atgaaggtga tggagttctt
     1021 tatgaaggtt tatggttatc gtggtaaaca tttgggtgga tcaaggaaac cagacggagc
     1081 aatttatact gtcggatctc ctattgatta cggtgtgatc gttgatacta aggcatattc
     1141 aggaggttat aatcttccaa ttggtcaagc agatgaaatg caaagatatg tcgaagagaa
     1201 tcaaacaaga aacaagcata tcaaccctaa tgaatggtgg aaagtctatc catcttcagt
     1261 aacagaattt aagttcttgt ttgtgagtgg tcatttcaaa ggaaactaca aagctcagct
     1321 tacaagattg aatcatatca ctaattgtaa tggagctgtt cttagtgtag aagagctttt
     1381 gattggtgga gaaatgatta aagctggtac attgacactt gaggaagtga gaaggaaatt
     1441 taataacggt gagataaact tttaatagga cgtccgatcg ttcaaacatt tggcaataaa
     1501 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     1561 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     1621 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     1681 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattgatcc
     1741 cccctcgaca gcttccggaa agggcgaatt cgcaactttg tatacaaaag ttgccgagct
     1801 cgcggccgca gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg
     1861 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     1921 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     1981 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2041 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2101 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2161 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2221 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2281 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2341 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     2401 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     2461 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     2521 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     2581 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     2641 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     2701 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     2761 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     2821 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     2881 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     2941 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3001 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg
     3061 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3121 gtgaaaaacc gcagcaggga ggcaaacaac gcagggaggc aaacaatgat atcacaactc
     3181 tcctgacgcg tcatcgtcgg ctacagcctc gggaattgct acctagctcg agcaagatcc
     3241 aaggagatat aacaatggct tcctcctgga ttgaacaaga tggattgcac gcaggttctc
     3301 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     3361 ctgatgccgc cgtgttccgg ctgtcagcgc agggtagacc ggttcttttt gtcaagaccg
     3421 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     3481 cgacgggcgt accttgcgct gctgtgctcg acgttgtcac tgaagcggga agggactggc
     3541 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     3601 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     3661 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3721 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     3781 ccaggctcaa ggcgagaatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3841 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3901 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     3961 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     4021 agcgcatcgc cttctatcgc cttcttgacg agttcttctg ataaccgcgg agagctcgaa
     4081 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     4141 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     4201 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     4261 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     4321 gtcatctatg ttactagatc gtgggcccgt ccagttccat tcgccctata gtgagtcgta
     4381 ttaaacctgc attaatgaat cggccaacgc gcggggctgc aggtcgacga cccagctttc
     4441 ttgtacaaag tgggagtgta cttcaagtca gtgggaaatc aataaaatga ttattttatg
     4501 aatatatttc attgtgcaag tagatagaaa ttacatatgt tacataacac acgaaataaa
     4561 caaaaaaaga caatccaaaa acaaacaccc caaaaaaaat aatcacttta gataaactcg
     4621 tatgaggaga ggcacgttca gtgactcgac gattcccgag caaaaaaagt ctccccgtca
     4681 cacatgtagt gggtgacgca attatcttta aagtaatcct tctgttgact tgtcattgat
     4741 aacatccagt cttcgtcagg attgcaaaga attatagaag ggatcccact cgagccaagc
     4801 ttggcgtgtc tgcaggtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
     4861 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
     4921 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
     4981 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
     5041 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     5101 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac
     5161 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt
     5221 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
     5281 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
     5341 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
     5401 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     5461 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     5521 gacccttcct ctatataagg aagttcattt catttggaga ggacctcgac tctagaggat
     5581 cccccttcct ctatataagg aagttcattt catttggaga ggtaagtttc acttcacaca
     5641 ttattactgt cttctaatac aaggtttttt atcaagctgg agaagagcat gatagtgggt
     5701 agtgccatct tgatgaagct cagaagcaac accaaggaag aaaataagaa aaggtgtgag
     5761 tttctcccag agaaactgga ataaatcatc tctttgagat gagcacttgg gataggtaag
     5821 gaaaacatat ttagattgga gtctgaagtt cttactagca gaaggcatgt tgttgtgact
     5881 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
     5941 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
     6001 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
     6061 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
     6121 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
     6181 caggtatttc cgttggatac gaattattcg tacgaccctc cctgcaggtc gactctagag
     6241 gatccccggg taccgagctc gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6481 ttgcgtattg gctagagcag cttgccaaca tggtggagca cgacactctc gtctactcca
     6541 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
     6601 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
     6661 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
     6721 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     6781 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg
     6841 tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa
     6901 gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc
     6961 cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc
     7021 atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag
     7081 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
     7141 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
     7201 cttcgcaaga ccttcctcta tataaggaag ttcatttcat ttggagagga cacgctgaaa
     7261 tcaccagtct ctctctacaa atctatctct ctcgagcttt cgcagatccg ggggggcaat
     7321 gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     7381 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     7441 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     7501 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     7561 acattgggga gtttagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     7621 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct acaaccggtc gcggaggcta
     7681 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     7741 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     7801 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     7861 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     7921 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     7981 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     8041 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     8101 cgccacgact ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     8161 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     8221 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     8281 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     8341 caaagaaata gagtagatgc cgacgcggat ctgtcgatcg acaagctcga gtttctccat
     8401 aataatgtgt gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg
     8461 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
     8521 aaatttctaa ttcctaaaac caaaatccag tactaaaatc cagatccccc gaattaattc
     8581 ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     8641 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     8701 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     8761 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     8821 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     8881 tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg
     8941 ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag
     9001 aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt
     9061 aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat
     9121 tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta
     9181 aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt
     9241 ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa
     9301 agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca
     9361 tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa
     9421 agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt
     9481 gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg
     9541 atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg
     9601 agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg
     9661 acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg
     9721 ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg
     9781 aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg
     9841 attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac ctagaatgca
     9901 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10021 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10081 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    10141 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10201 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10261 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10321 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    10381 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    10441 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10501 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10561 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10621 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    10681 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    10741 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
    10801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
    10861 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
    10921 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
    10981 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
    11041 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
    11101 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
    11161 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
    11221 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
    11281 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
    11341 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
    11401 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
    11461 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
    11521 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
    11581 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
    11641 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
    11701 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
    11761 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
    11821 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
    11881 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
    11941 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
    12001 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
    12061 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
    12121 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
    12181 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
    12241 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
    12301 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
    12361 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
    12421 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
    12481 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
    12541 ggccggatgg tttgcgaccg tcacgccgat tcctcgggct tgggggttcc agtgccattg
    12601 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    12661 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    12721 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    12781 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    12841 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    12901 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    12961 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    13021 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    13081 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    13141 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    13201 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    13261 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    13321 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    13381 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    13441 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    13501 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    13561 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    13621 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    13681 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    13741 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    13801 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    13861 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    13921 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    13981 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    14041 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    14101 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    14161 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    14221 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    14281 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    14341 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    14401 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    14461 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    14521 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    14581 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    14641 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    14701 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    14761 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    14821 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    14881 tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
    14941 ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag
    15001 ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    15061 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    15121 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatg
//
