LOCUS       sequence-78602-e        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression vector for yeast, N152,C18 architecture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY566
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..28
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9..27
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(75..95)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(75..93)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(114..130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(127..149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    138..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(162..168,169..186,187..192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    207..228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             279..866
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcc
       61 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      121 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      181 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      241 tcactgcccg ctttccaccg gtcgtctcca acggatccca gctagtgaaa tctgaattgg
      301 aagagaagaa atctgaactt agacataaat tgaaatatgt gccacatgaa tatattgaat
      361 tgattgaaat cgcaagaaat tcaactcagg atagaatcct tgaaatgaag gtgatggagt
      421 tctttatgaa ggtttatggt tatcgtggta aacatttggg tggatcaagg aaaccagacg
      481 gagcaattta tactgtcgga tctcctattg attacggtgt gatcgttgat actaaggcat
      541 attcaggagg ttataatctt ccaattggtc aagcagatga aatgcaaaga tatgtcgaag
      601 agaatcaaac aagaaacaag catatcaacc ctaatgaatg gtggaaagtc tatccatctt
      661 cagtaacaga atttaagttc ttgtttgtga gtggtcattt caaaggaaac tacaaagctc
      721 agcttacaag attgaatcat atcactaatt gtaatggagc tgttcttagt gtagaagagc
      781 ttttgattgg tggagaaatg attaaagctg gtacattgac acttgaggaa gtgagaagga
      841 aatttaataa cggtgagata aacttttaat aggagctcga atttccccga tcgttcaaac
      901 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
      961 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
     1021 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
     1081 aaaatatagc gcgcaaacta ggataaatta tc
//
