LOCUS       sequence-78609-e        1079 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN expression vector for yeast, N152,C18 architecture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, 
            Voytas DF
  TITLE     TALENs enable efficient plant genome engineering.
  JOURNAL   Plant Physiol. 2012 Nov 2.
  PUBMED    23124327
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2012 Nov 2."
COMMENT     Sequence Label: pZHY565
FEATURES             Location/Qualifiers
     source          1..1079
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..29
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10..28
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(76..96)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(76..94)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(115..131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(115..131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(128..150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    139..155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(163..169,170..187,188..193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    208..229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             280..867
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gagcgcgcgt aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc
       61 ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
      121 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      241 ctcactgccc gctttccacc ggtcgtctcc aacggatccc agctagtgaa atctgaattg
      301 gaagagaaga aatctgaact tagacataaa ttgaaatatg tgccacatga atatattgaa
      361 ttgattgaaa tcgcaagaaa ttcaactcag gatagaatcc ttgaaatgaa ggtgatggag
      421 ttctttatga aggtttatgg ttatcgtggt aaacatttgg gtggatcaag gaaaccagac
      481 ggagcaattt atactgtcgg atctcctatt gattacggtg tgatcgttga tactaaggca
      541 tattcaggag gttataatct tccaattggt caagcagatg aaatgcaaag atatgtcgaa
      601 gagaatcaaa caagaaacaa gcatatcaac cctaatgaat ggtggaaagt ctatccatct
      661 tcagtaacag aatttaagtt cttgtttgtg agtggtcatt tcaaaggaaa ctacaaagct
      721 cagcttacaa gattgaatca tatcactaat tgtaatggag ctgttcttag tgtagaagag
      781 cttttgattg gtggagaaat gattaaagct ggtacattga cacttgagga agtgagaagg
      841 aaatttaata acggtgagat aaacttttaa taggagctcg aatttccccg atcgttcaaa
      901 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
      961 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     1021 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaa
//
