LOCUS       sequence-78611-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pCDM4-GLY
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             519..563
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     601..620
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        601..619
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    620..644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             659..681
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cctttatcgc ggcacaaggc cacgatgtgg gtaaatccct gtacgaagct gacctggttg
       61 acgaagctaa acgtctgctg accacctgca acatcccggt tccgtctgat gttcgcgtag
      121 caaccgagtt ctctgaaact gcaccggcta ccctgaaatc tgttaacgat gtgaaagctg
      181 acgagcagat cctggatatc ggtgatgctt ccgctcagga actggctgaa atcctgaaga
      241 atgcgaaaac cattctgtgg aacggtccgg ttggcgtgtt cgaattcccg aacttccgca
      301 aaggtactga aatcgtggct aacgctatcg cagacagcga agcgttctcc atcgctggcg
      361 gcggcgacac tctggcagca atcgacctgt tcggcattgc tgacaaaatc tcctacatct
      421 ccactggcgg cggcgcattc ctcgaattcg tggaaggtaa agtactgcct gcagtagcga
      481 tgctcgaaga gcgcgctaag aagtaactcg agtctggtaa agaaaccgct gctgcgaaat
      541 ttgaacgcca gcacatggac tcgtctacta ggatcgagat cgatctcgat cccgcgaaat
      601 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      661 tgtttaactt taagaaggag atatacagga ggcatatgac tatcaaagta ggtatcaacg
      721 gttttggccg tatcggtcgc attgttttcc gtgctgctca gaaacgttct gacatcgaga
      781 tcgttgcaat caacgacctg ttagacgctg attacatggc atacatgctg aaatatgact
      841 ccactcacgg ccgtttcgac ggtaccgttg aagtgaaaga cggtcatctg at
//
