LOCUS       sequence-78621-e        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS module plasmid for Cermak, et al., 2011 TALEN kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  TITLE     module plasmid for Cermak, et al., 2011 TALEN kit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNS9
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(11..27)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11..27)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      313..901
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     802..821
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1029..1034,1035..1051,1052..1057)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
       61 ttacattgca caagataaaa atatatcatc atgcctcctc tagaggtctc ctcgaaacgg
      121 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
      181 ctatcgccag caacagcggc ggcaagcaag cgctcggaga ccctcgagcc acccatgacc
      241 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
      301 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
      361 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
      421 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
      481 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
      541 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
      601 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
      661 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
      721 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
      781 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
      841 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
      901 aaacgccagc aacagcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      961 tgttctttcc tgcgttatcc acagaatcag gggataacgc aggaaagaac atgaattaat
     1021 tctcatgttt gacagcttat catcgattag ctttaatgcg gtagtttatc acagttaaat
     1081 tgctaacgca gtcagg
//
