LOCUS       sequence-78627-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS module plasmid for Cermak, et al., 2011 TALEN kit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  TITLE     module plasmid for Cermak, et al., 2011 TALEN kit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNS3
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(17..33)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17..33)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      318..906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     807..826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
       61 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ggtctcagga
      121 ccatggcctg accccggacc aagtggtggc tatcgccagc aacagcggcg gcaagcaagc
      181 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag ggagaccctc gagccaccca
      241 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
      301 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      361 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      421 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      481 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      541 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      601 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      661 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      721 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      781 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      841 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      901 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      961 cacatgttct ttcctgcgtt atcccctgat tctgtggata acgcaggaaa gaacatgaat
     1021 taattctcat gtttgacagc ttatcatcga ttagcttt
//
