LOCUS       sequence-78634-e       15212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single component LSL vector. Contains 2x35S:Zif268:FokI followed by 
            the us:NPTII template. This sequence is between the LIR and SIR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15212)
  AUTHORS   Baltes NJ, Gil-Humanes J, Cermak T, Atkins PA, Voytas DF
  TITLE     DNA Replicons for Plant Genome Engineering.
  JOURNAL   Plant Cell. 2014 Jan;26(1):151-63. doi: 10.1105/tpc.113.119792
  PUBMED    24443519
REFERENCE   2  (bases 1 to 15212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1105/tpc.113"; 
            journalName: "Plant Cell"; date: "2014-01"; volume: "26"; issue: 
            "1"; pages: "151-63"
COMMENT     Sequence Label: pLSLZ.D.R
FEATURES             Location/Qualifiers
     source          1..15212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1129..1323
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(1559..1581)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1667..1807
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1722..1741
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1822..1839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2668..3462)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    3887..3911
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    3989..4163
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(5253..5930)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        6833..7177
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7237..7261
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7295..7315
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7586..8170
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="LVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKVM
                     EFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQRY
                     VEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVLS
                     VEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      8184..8436
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8481..8501
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             10008..10799
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10059..10078)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10669..10688
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      10826..11078
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(11102..11121)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11103..11121)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    12821..12845
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14145..14774
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(15208..15212,1..1063)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
ORIGIN
        1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
       61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
      121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
      181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
      241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
      301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
      361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
      421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
      481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
      541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
      601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
      661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
      721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
      781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
      841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
      901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
      961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
     1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
     1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
     3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
     3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
     4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
     4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
     4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
     4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
     5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
     5221 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
     5281 cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
     5341 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
     5401 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
     5461 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
     5521 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
     5581 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
     5641 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
     5701 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
     5761 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
     5821 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
     5881 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
     5941 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
     6001 tgtcgactat cggacgagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
     6061 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
     6121 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
     6181 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
     6241 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
     6301 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
     6361 caggtatttc cgttggatac gaattattcg tacgaccctc ggcgcgtcca agcttggcgt
     6421 gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
     6481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
     6541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
     6601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
     6661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     6721 ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag cacgacacac
     6781 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
     6841 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
     6901 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
     6961 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
     7021 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
     7081 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
     7141 cctctatata aggaagttca tttcatttgg agaggacctc gactctagag gatccccggg
     7201 taccgggccc cccctcgagg cgcgccaagc tatcaaacaa gtttgtacaa aaaagcaggc
     7261 tccgaattcg cccttcacca tggcttcctc ccctccaaag aaaaagagaa aggttcttga
     7321 attgccttac gcttgtccag ttgagtcttg tgatagacgt tttagtaggt cagacgaact
     7381 cactaggcat attagaatcc acacaggaca gaagcctttc cagtgtagaa tctgtatgag
     7441 gaactttagt cgtagcgatc atttgactac acatatcaga acccatactg gtgagaaacc
     7501 attcgcatgt gacatctgtg gtcgtaagtt cgcaagaagt gatgaaagga agcgtcatac
     7561 aaagatccat cttcgtcaga aggaactagt gaaatctgaa ttggaagaga agaaatctga
     7621 acttagacat aaattgaaat atgtgccaca tgaatatatt gaattgattg aaatcgcaag
     7681 aaattcaact caggatagaa tccttgaaat gaaggtgatg gagttcttta tgaaggttta
     7741 tggttatcgt ggtaaacatt tgggtggatc aaggaaacca gacggagcaa tttatactgt
     7801 cggatctcct attgattacg gtgtgatcgt tgatactaag gcatattcag gaggttataa
     7861 tcttccaatt ggtcaagcag atgaaatgca aagatatgtc gaagagaatc aaacaagaaa
     7921 caagcatatc aaccctaatg aatggtggaa agtctatcca tcttcagtaa cagaatttaa
     7981 gttcttgttt gtgagtggtc atttcaaagg aaactacaaa gctcagctta caagattgaa
     8041 tcatatcact aattgtaatg gagctgttct tagtgtagaa gagcttttga ttggtggaga
     8101 aatgattaaa gctggtacat tgacacttga ggaagtgaga aggaaattta ataacggtga
     8161 gataaacttt taataggacg tccgatcgtt caaacatttg gcaataaagt ttcttaagat
     8221 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     8281 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     8341 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     8401 aattatcgcg cgcggtgtca tctatgttac tagatcggga attgatcccc cctcgacagc
     8461 ttccggaaag ggcgaattcg caactttgta tacaaaagtt gccgagcttt gggtacgtca
     8521 cgtggctcga gcgcgtagtc ctcggtaagc agtcttactt ccatgatttc tttaactatg
     8581 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca
     8641 ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg
     8701 ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac
     8761 aaggcactag cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt
     8821 atctctatga actgtgcgtc acagccaaaa gccagacaga gtgtgatatc tacccgcttc
     8881 gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt
     8941 tctactttac tggctttggt cgtcatgaag atgcggactt gcgtggcaaa ggattcgata
     9001 acgtgctgat ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta
     9061 cctcgcatta cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg
     9121 tgattgatga aactgctgct gtcggcttta acctctcttt aggcattggt ttcgaagcgg
     9181 gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc
     9241 acttacaggc gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt
     9301 ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac
     9361 tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt
     9421 tctgcgacgc tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt
     9481 attacggatg gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag
     9541 aacttctggc ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg
     9601 atacgttagc cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg
     9661 catggctgga tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg
     9721 tatggaattt cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga
     9781 aagggatctt cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct
     9841 ggactggcat gaacttcggt gaaaaaccgc agcagggagg caaacaacgc agggaggcaa
     9901 acaatgatat cacaactctc ctgacgcgtc atcgtcggct acagcctcgg gaattgctac
     9961 ctagctcgag caagatccaa ggagatataa caatggcttc ctcctggatt gaacaagatg
    10021 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
    10081 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag ggtagaccgg
    10141 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
    10201 ggctatcgtg gctggccacg acgggcgtac cttgcgctgc tgtgctcgac gttgtcactg
    10261 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
    10321 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
    10381 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
    10441 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
    10501 cgccagccga actgttcgcc aggctcaagg cgagaatgcc cgacggcgag gatctcgtcg
    10561 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
    10621 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
    10681 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
    10741 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat
    10801 aaccgcggag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
    10861 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
    10921 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
    10981 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
    11041 taaattatcg cgcgcggtgt catctatgtt actagatcgt gggcccgtcc agttccattc
    11101 gccctatagt gagtcgtatt aaacctgcat taatgaatcg gccaacgcgc ggggagaggc
    11161 ggtttgcgta ttgagtgtac ttcaagtcag tgggaaatca ataaaatgat tattttatga
    11221 atatatttca ttgtgcaagt agatagaaat tacatatgtt acataacaca cgaaataaac
    11281 aaaaaaagac aatccaaaaa caaacacccc aaaaaaaata atcactttag ataaactcgt
    11341 atgaggagag gcacgttcag tgactcgacg attcccgagc aaaaaaagtc tccccgtcac
    11401 acatgtagtg ggtgacgcaa ttatctttaa agtaatcctt ctgttgactt gtcattgata
    11461 acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg gatcccacct tttattttct
    11521 tcttttttcc atatttaggg ttgacagtga aatcagactg gcaacctatt aattgcttcc
    11581 acaatgggac gaacttgaag gggatgtcgt cgatgatatt ataggtggcg tgttcatcgt
    11641 agttggtgaa atcgatggta ccgttccaat agttgtgtcg tccgagactt ctagcccagg
    11701 tggtctttcc ggtacgagtt ggtccgcaga tgtagaggct ggggtgtcgg attccattcc
    11761 ttccattgtc cttgttaaat cggccatcca ttcaaggtca gattgagctt gttggtatga
    11821 gacaggatgt atgtaagtat aagcgtctat gcttacatgg tatagatggg tttccctcca
    11881 ggagtgtaga tcttcgtggc agcgaagatc tgattctgtg aagggcgaca catacggttc
    11941 aggttgtgga gggaataatt tgttggctga atattccagc cattgaagct ttgttgccca
    12001 ttcatgaggg aattcttcct tgatcatgtc aagatattcc tccttagacg ttgcagtctg
    12061 gataatagtt ctccatcgtg cgtcagattt gcgaggagaa accttatgat ctcggaaatc
    12121 tcctctggtt ttaatatctc cgtcctttga tatgtaatca aggacttgtt tagagtttct
    12181 agctggctgg atattagggt gatttccttc aaaatcgaaa aaagaaggat ccctaataca
    12241 aggtttttta tcaagctgga gaagagcatg atagtgggta gtgccatctt gatgaagctc
    12301 agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt ttctcccaga gaaactggaa
    12361 taaatcatct ctttgagatg agcacttggg ataggtaagg aaaacatatt tagattggag
    12421 tctgaagttc ttactagcag aaggcatgtt gttgtgactc cgaggggttg cctcaaactc
    12481 tatcttataa ccggcgtgga ggcatggagg caggggtatt ttggtcattt taatagatag
    12541 tggaaaatga cgtggaattt acttaaagac gaagtctttg cgacaagggg gggcccacgc
    12601 cgaatttaat attaccggcg tggccccccc ttatcgcgag tgctttagca cgagcggtcc
    12661 agatttaaag tagaaaattt cccgcccact agggttaaag gtgttcacac tataaaagca
    12721 tatacgatgt gatggtattt gatggagcgt atattgtatc aggtatttcc gttggatacg
    12781 aattattcgt acgaccctca tagtttaaac tatcagtgtt tgacaggata tattggcggg
    12841 taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg cgtgaaaagg
    12901 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
    12961 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
    13021 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
    13081 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
    13141 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
    13201 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
    13261 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
    13321 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
    13381 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
    13441 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
    13501 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
    13561 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
    13621 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
    13681 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
    13741 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
    13801 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
    13861 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
    13921 tacgtgttcg agccgcccgc gcacggctca accgtgcggc tgcatgaaat cctggccggt
    13981 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
    14041 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
    14101 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
    14161 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
    14221 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
    14281 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
    14341 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
    14401 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
    14461 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
    14521 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
    14581 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
    14641 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
    14701 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
    14761 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
    14821 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
    14881 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
    14941 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
    15001 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
    15061 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
    15121 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
    15181 cccccaagcc cgaggaatcg gcgtgacggt cg
//
